張欣暘 副教授

專任副教授
張欣暘
Hsin-Yang Changoid
phone 02-2826-7168／lab: ext. 65789 or 65771 or 65726
mail hychang5@nycu.edu.tw
address 傳統醫學大樓甲棟1樓R108
  • Aug. 2020-present 國立陽明交通大學 生命科學系暨基因體科學研究所 副教授
  • Aug. 2019-Jul. 2020 國立中山大學 海洋生物科技暨資源學系 副教授
  • Aug. 2015- Jul. 2019 國立中山大學 海洋生物科技暨資源學系 助理教授
2010 美國伊利諾大學香檳分校 (University of Illinois at Urbana-Champaign) 生物化學所 博士
結構生物學 (Structural biology)、X-Ray蛋白晶體學(X-ray crystallography)、小角度散射 (SAXS: Small-angle X-ray scattering)、酵素動力學 (Enzyme kinetics)
  • 生物化學
  • 生命科學總論
  • 結構生物學
(1)實驗室研發如何利用酵素生合成天然抗生素如Vitroprocines。我們目前發現其生合成反應機制需要兩個特別的酵素VsAOS-2和VsOR 來完成。除了生合成途徑，我們也專注於此類酵素在分子層次及三級結構之功能分析。此類抗生素可對抗多重抗藥性 Acinetobacter baumannii (鮑氏不動桿菌) 等在醫院常被感染之致病菌，如：肺炎、腦膜炎、皮膚組織感染及泌尿道感染。這項研究有助於新型抗生素研發。
 
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(2)實驗室研究細胞膜蛋白如何進入生物膜的生理機制。大多數真核細胞膜蛋白質首先被插入內質網， 此過程稱為共同轉譯途徑 (co-translational pathway)。然而，有一類在生理功能上比較特別的膜蛋白，屬於尾端固定（tail-anchored, TA）膜蛋白家族，而是經由另一個稱為後轉譯途徑 (post-translational pathway) 的特殊新途徑完成。此途徑只有在真核細胞才有。錯誤的TA運送會導致癌症、糖尿病和心臟病等相關人類疾病。我們以真核物種為研究對象，探討TA 膜蛋白運如何被ArsAs (或Get3) 運輸蛋白送至各胞器外膜。這項研究對於TA 膜蛋白所引發的致病分子機制將有更進一步了解。
 
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期刊發表
  1. SWA Permanasari, HC Lin, JY Lin, MX Hong, HY Chang, WD He, LH Lo, YL Yang, TM Lee, and CC Liaw (2026) Journal of Agricultural and Food Chemistry 2026 74 (13), 10747-10758, DOI: 10.1021/acs.jafc.5c08939
  2. SY Cheng, YJ Chen, HC Lin, HY Chang, MD Huang (2025) Genetic Analysis of Polyunsaturated Fatty Acids Biosynthesis Pathway Determines Four Distinct Thraustochytrid Types. Environmental Microbiology, 27:e70090.
  3. CC Chen, YR Huang, YT Chan, HY Lin, HJ Lin, CD Hsiao, TP Ko, TW Lin, YH Lan, HY Lin, HY Chang*(2024) A distinct dimer configuration of a diatom Get3 forming a tetrameric complex with its tail-anchored membrane cargo.BMC Biology 23:136.
  4. MD Huang, CW Wu, HY Chou, SY Cheng and HY Chang*(2023) The revealing of a novel lipid transfer protein lineage in green algae. BMC Plant Biology 23:21.
  5. CC Liaw, LH Lo, TH Cheng, YT Chan, YR Huang, AHJ Wang, HY Chang*(2022) Biosynthesis of Vitroprocines by Identified α-oxoamine Synthase and Oxidoreductase from Vibrio sp. QWI-06, ACS Organic Letters.
  6. HY Chang*, LH Lo, YH Lan, MX Hong, YT Chan, TP Ko, YR Huang, TH Cheng, CC Liaw (2021) Structural insights into the substrate selectivity of α-oxoamine synthases from marine Vibrio sp. QWI-06, Colloids Surf. B Biointerfaces.
  7. HY Chang*, TH Cheng, AHJ Wang (2020) Structure, Catalysis and Inhibition Mechanism of Prenyltransferase (Critical Review). IUBMB Life
  8. HC Lin, WH Li, CC Chen, TH Cheng, YH Lan, MD Huang, WM Chen, JS Chang, HY Chang*(2020) Diverse enzymes with industrial applications in four thraustochytrid genera. Front. Microbiol.
  9. CY Chiang, CC Chou, HY Chang, MF Hsu, PJ Pao, MH Chiang, AHJ Wang (2020) Biochemical and molecular dynamics studies of archaeal polyisoprenyl pyrophosphate phosphatase from Saccharolobus solfataricus. Enzyme Microb. Technol. doi.org/10.1016/ j.enzmictec.2020.109585
  10. HL Yeh, TH Lin, CC Chen, TX Cheng, HY Chang, TM Lee (2019) Monodehydroascorbate reductase plays a role in the tolerance of Chlamydomonas reinhardtii to photooxidative stress. Plant Cell Physiol. 60: 2167–2179.
  11. TW Lin, CC Chen, SM Wu, YC Chang, YC Li, YW Su, CD Hsiao, HY Chang*. (2019) Structural analysis of chloroplast tail-anchored membrane protein recognition by ArsA1, The Plant Journal, 99:128-143.
  12. HY Chang*, TP Ko, YC Chang, KF Huang, CY Lin, HY Chou, CY Chiang, HJ Tsai. (2019) Crystal structure of the blue fluorescent protein with a Leu-Leu-Gly tri-peptide chromophore derived from the purple chromoprotein of Stichodactyla haddoni, Int. J. Biol. Macromol. 130: 675-684.
  13. HY Chang*, ST Lin, TP Ko, SM Wu, TH Lin, YC Chang, KF Huang, TM Lee. (2017) Enzymatic characterization and crystal structure analysis of Chlamydomonas reinhardtii dehydroascorbate reductase and their implications for oxidative stress, Plant Physiol. Biochem. 120: 144-155.
  14. M Maestre-Reyna, SM Wu, YC Chang, CC Chen, A Maestre-Reyna, AHJ Wang, HY Chang*. (2017) In search of tail-anchored protein machinery in plants: reevaluating the role of arsenite transporters, Scientific Report 7:46022 | DOI: 10.1038/srep46022.
  15. HY Chang, CC Chou, ML Wu, AHJ Wang. (2017) Expression, purification and enzymatic characterization of undecaprenyl pyrophosphate phosphatase from Vibrio vulnificus, Protein Expr. Purif. 133: 121-131.
  16. C von Ballmoos, I Smirnova, F Poiana, N Gonska, HY Chang, RB Gennis, P Brzezinski, P Ädelroth. (2017) Dynamics of the KB proton pathway in cytochrome ba3 from Thermus thermophiles, Isr. J. Chem. 57: 1-14. DOI: 10.1002/ijch.201600136.
  17. ST Lin, CW Chiou, YL Chu, YH, YF Tseng, YC Chen, HJ Chen, HY Chang, TM Lee. (2016) Enhanced ascorbate regeneration via dehydroascorbate reductase confers tolerance to photo-oxidative stress in Chlamydomonas reinhardtii. Plant Cell Physiol. 10: 2104-2121.
  18. A Mateja, M Paduch, HY Chang, A Szydlowska, AA Kossiakoff, RS Hegde, RJ Keenan. (2015) Structure of the Get3 targeting factor in complex with its membrane protein cargo. Science. 347: 1152-1155.
  19. HY Chang, CC Chou, MF Hsu, AHJ Wang. (2014) Proposed carrier lipid-binding site of undecaprenyl pyrophosphate phosphatase from Escherichia coli. J. Biol. Chem. 289: 18719-18735.
  20. I Smirnova, HY Chang, C von Ballmoos, P Ädelroth, RB Gennis, P Brzezinski. (2013) Single mutations that redirect internal proton transfer in the ba3 oxidase from Thermus thermophilus. Biochemistry 52: 7022-7030.
  21. HY Chang, SK Choi, AS Vakkasoglu, Y Chen, J Hemp, JA Fee, RB Gennis. (2012) Exploring the proton pump and exit pathway for pumped protons in cytochrome ba3 from Thermus thermophilus. Proc Natl Acad Sci U S A 109: 5259-5264.
  22. HY Chang, Y Ahn, LA Pace, MT Lin, YH Lin, RB Gennis. (2010) The diheme cytochrome c4 from Vibrio cholerae is a natural electron donor to the cbb3-type cytochrome c oxidase. Biochemistry 49: 7494-7503.
  23. I Smirnova, J Reimann, C von Ballmoos, HY Chang, RB Gennis, JA Fee, P Brzezinski, P Ädelroth. (2010) Functional role of Thr-312 and Thr-315 in the proton-transfer pathway in ba3 cytochrome c oxidase from Thermus thermophilus. Biochemistry 49: 7033-7049.
  24. HY Chang, J Hemp, Y Chen, JA Fee, RB Gennis. (2009) The cytochrome ba3 oxygen reductase from Thermus thermophilus uses a single input channel for proton delivery to the active site and for proton pumping. Proc Natl Acad Sci U S A 106: 16169-16173.
  25. TH Kuo, SC Chuang, SY Chang, PH Liang. (2006) Ligand specificities and structural requirements of two Tachypleus plasma lectins for bacterial trapping. Biochem. J. 393: 757-766.
  26. APC Chen, SY Chang, YC Lin, YS Sun, CT Chen, AHJ Wang, PH Liang. (2005) Substrate and product specificities for cis-type undecaprenyl pyrophosphate synthase. Biochem. J. 386: 169-176.
  27. SY Chang, TP Ko, APC Chen, AHJ Wang, PH Liang. (2004) Substrate binding mode and reaction mechanism of undecaprenyl pyrophosphate synthase deduced from crystallographic studies. Protein Sci. 13: 971-978.
  28. SY Chang, TP Ko, PH Liang, AHJ Wang. (2003) Catalytic mechanism revealed by the crystal structure of undecaprenyl pyrophosphate synthase in complex with sulfate, magnesium, and Triton. J. Biol. Chem. 278: 29298-29307.
  29. SY Chang, YK Chen, AHJ Wang, PH Liang. (2003) Identification of the active conformation and the importance of length of the flexible loop 72-83 in regulating the conformational change of undecaprenyl pyrophosphate synthase. Biochemistry. 42: 14452-14459.
  30. CE Atreya, EF Johnson, J Williamson, SY Chang, PH Liang, KS Anderson. (2003). Probing electrostatic channeling in protozoal bifunctional thymidylate synthase-dihydrofolate reductase using site-directed mutagenesis. J. Biol. Chem. 278: 28901- 28911.
  31. SY Chang, PC Tsai, CS Tseng, PH Liang. (2001) Refolding and characterization of a yeast dehydrodolichyl diphosphate synthase overexpressed in Escherichia coli. Prot. Exp. Puri. 23: 432-43.
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