張欣暘 副教授<![CDATA[<div class="ed_model01 clearfix">
<div class="ed_txt"><span style="font-size:110%;">專任副教授</span></div>
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<div class="ed_pic_left"><span style="font-size:110%;"><img alt="張欣暘" src="/userfiles/dlsch/images/20260317112002099.jpg"></span></div>
<div class="ed_title01"><span style="font-size:110%;">Hsin-Yang Chang<img alt="oid" src="/userfiles/dlsch/images/20260316090416127.png"></span></div>
<div class="ed_txt"><span style="font-size:110%;"><img alt="phone" src="/userfiles/dlsch/images/20260316113711693.png"> 02-2826-7168／lab: ext. 65789 or 65771 or 65726<br>
<img alt="mail" src="/userfiles/dlsch/images/20260316113732276.png"> <a href="mailto:hychang5@nycu.edu.tw" id="1-2" title="hychang5@nycu.edu.tw">hychang5@nycu.edu.tw</a><br>
<img alt="address" src="/userfiles/dlsch/images/20260316113722447.png"> 傳統醫學大樓甲棟1樓R108</span></div>
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<li><span style="font-size:110%;">Aug. 2020-present 國立陽明交通大學 生命科學系暨基因體科學研究所 副教授</span></li>
<li><span style="font-size:110%;">Aug. 2019-Jul. 2020 國立中山大學 海洋生物科技暨資源學系 副教授</span></li>
<li><span style="font-size:110%;">Aug. 2015- Jul. 2019 國立中山大學 海洋生物科技暨資源學系 助理教授</span></li>
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<div><span style="font-size:110%;">2010 美國伊利諾大學香檳分校 (University of Illinois at Urbana-Champaign) 生物化學所 博士</span></div>
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<div><span style="font-size:110%;">結構生物學 (Structural biology)、X-Ray蛋白晶體學(X-ray crystallography)、小角度散射 (SAXS: Small-angle X-ray scattering)、酵素動力學 (Enzyme kinetics)</span></div>
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<li><span style="font-size:110%;">生物化學</span></li>
<li><span style="font-size:110%;">生命科學總論</span></li>
<li><span style="font-size:110%;">結構生物學</span></li>
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<div><span style="font-size:110%;">(1)實驗室研發如何利用酵素生合成天然抗生素如Vitroprocines。我們目前發現其生合成反應機制需要兩個特別的酵素VsAOS-2和VsOR 來完成。除了生合成途徑，我們也專注於此類酵素在分子層次及三級結構之功能分析。此類抗生素可對抗多重抗藥性 Acinetobacter baumannii (鮑氏不動桿菌) 等在醫院常被感染之致病菌，如：肺炎、腦膜炎、皮膚組織感染及泌尿道感染。這項研究有助於新型抗生素研發。</span><br>
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<div style="text-align: center;"><span style="font-size:110%;"><img alt="chang" src="/userfiles/dlsch/images/20260317112438671.png"></span><br>
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<div><span style="font-size:110%;">(2)實驗室研究細胞膜蛋白如何進入生物膜的生理機制。大多數真核細胞膜蛋白質首先被插入內質網， 此過程稱為共同轉譯途徑 (co-translational pathway)。然而，有一類在生理功能上比較特別的膜蛋白，屬於尾端固定（tail-anchored, TA）膜蛋白家族，而是經由另一個稱為後轉譯途徑 (post-translational pathway) 的特殊新途徑完成。此途徑只有在真核細胞才有。錯誤的TA運送會導致癌症、糖尿病和心臟病等相關人類疾病。我們以真核物種為研究對象，探討TA 膜蛋白運如何被ArsAs (或Get3) 運輸蛋白送至各胞器外膜。這項研究對於TA 膜蛋白所引發的致病分子機制將有更進一步了解。</span><br>
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<div style="text-align: center;"><span style="font-size:110%;"><img alt="chang" src="/userfiles/dlsch/images/20260317112511470.jpg"></span></div>
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<div><span style="font-size:110%;"><strong>期刊發表</strong></span></div>
<ol>
<li>SWA Permanasari, HC Lin, JY Lin, MX Hong, <u><strong>HY Chang</strong></u>, WD He, LH Lo, YL Yang, TM Lee, and CC Liaw (2026) Journal of Agricultural and Food Chemistry 2026 74 (13), 10747-10758, DOI: 10.1021/acs.jafc.5c08939</li>
<li><span style="font-size:110%;">SY Cheng, YJ Chen, HC Lin, <strong><u>HY Chang</u></strong>, MD Huang (2025) <a href="https://enviromicro-journals.onlinelibrary.wiley.com/doi/10.1111/1462-2920.70090" id="1-3" rel="noreferrer noopener" target="_blank" title="Genetic Analysis of Polyunsaturated Fatty Acids Biosynthesis Pathway Determines Four Distinct Thraustochytrid Types.(開啟新視窗)">Genetic Analysis of Polyunsaturated Fatty Acids Biosynthesis Pathway Determines Four Distinct Thraustochytrid Types.</a> Environmental Microbiology, 27:e70090.</span></li>
<li><span style="font-size:110%;">CC Chen, YR Huang, YT Chan, HY Lin, HJ Lin, CD Hsiao, TP Ko, TW Lin, YH Lan, HY Lin, <strong><u>HY Chang*</u></strong>(2024) <a href="https://link.springer.com/article/10.1186/s12915-024-01933-x" id="1-4" rel="noreferrer noopener" target="_blank" title="A distinct dimer configuration of a diatom Get3 forming a tetrameric complex with its tail-anchored membrane cargo(開啟新視窗)">A distinct dimer configuration of a diatom Get3 forming a tetrameric complex with its tail-anchored membrane cargo</a>.BMC Biology 23:136.</span></li>
<li><span style="font-size:110%;">MD Huang, CW Wu, HY Chou, SY Cheng and <strong><u>HY Chang*</u></strong>(2023) <a href="https://link.springer.com/article/10.1186/s12870-023-04040-1" id="1-5" rel="noreferrer noopener" target="_blank" title="The revealing of a novel lipid transfer protein lineage in green algae(開啟新視窗)">The revealing of a novel lipid transfer protein lineage in green algae</a>. BMC Plant Biology 23:21.</span></li>
<li><span style="font-size:110%;">CC Liaw, LH Lo, TH Cheng, YT Chan, YR Huang, AHJ Wang, <strong><u>HY Chang*</u></strong>(2022) <a href="https://pubs.acs.org/doi/10.1021/acs.orglett.2c01178" id="1-6" rel="noreferrer noopener" target="_blank" title="Biosynthesis of Vitroprocines by Identified α-oxoamine Synthase and Oxidoreductase from Vibrio sp. QWI-06(開啟新視窗)">Biosynthesis of Vitroprocines by Identified α-oxoamine Synthase and Oxidoreductase from Vibrio sp. QWI-06</a>, ACS Organic Letters.</span></li>
<li><span style="font-size:110%;"><strong><u>HY Chang*</u></strong>, LH Lo, YH Lan, MX Hong, YT Chan, TP Ko, YR Huang, TH Cheng, CC Liaw (2021) <a href="https://www.sciencedirect.com/science/article/pii/S0927776521006706?via%3Dihub" id="1-7" rel="noreferrer noopener" target="_blank" title="Structural insights into the substrate selectivity of α-oxoamine synthases from marine Vibrio sp. QWI-06(開啟新視窗)">Structural insights into the substrate selectivity of α-oxoamine synthases from marine Vibrio sp. QWI-06</a>, Colloids Surf. B Biointerfaces.</span></li>
<li><span style="font-size:110%;"><strong><u>HY Chang*</u></strong>, TH Cheng, AHJ Wang (2020) <a href="https://iubmb.onlinelibrary.wiley.com/doi/10.1002/iub.2418" id="1-8" rel="noreferrer noopener" target="_blank" title="Structure, Catalysis and Inhibition Mechanism of Prenyltransferase(開啟新視窗)">Structure, Catalysis and Inhibition Mechanism of Prenyltransferase</a> (Critical Review). IUBMB Life</span></li>
<li><span style="font-size:110%;">HC Lin, WH Li, CC Chen, TH Cheng, YH Lan, MD Huang, WM Chen, JS Chang, <strong><u>HY Chang*</u></strong>(2020) <a href="https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2020.573907/full" id="1-9" rel="noreferrer noopener" target="_blank" title="Diverse enzymes with industrial applications in four thraustochytrid genera.(開啟新視窗)">Diverse enzymes with industrial applications in four thraustochytrid genera.</a> Front. Microbiol.</span></li>
<li><span style="font-size:110%;">CY Chiang, CC Chou, <strong><u>HY Chang</u></strong>, MF Hsu, PJ Pao, MH Chiang, AHJ Wang (2020) Biochemical and molecular dynamics studies of archaeal polyisoprenyl pyrophosphate phosphatase from Saccharolobus solfataricus. Enzyme Microb. Technol. doi.org/10.1016/ j.enzmictec.2020.109585</span></li>
<li><span style="font-size:110%;">HL Yeh, TH Lin, CC Chen, TX Cheng, <strong><u>HY Chang</u></strong>, TM Lee (2019) Monodehydroascorbate reductase plays a role in the tolerance of Chlamydomonas reinhardtii to photooxidative stress. Plant Cell Physiol. 60: 2167–2179.</span></li>
<li><span style="font-size:110%;">TW Lin, CC Chen, SM Wu, YC Chang, YC Li, YW Su, CD Hsiao, <strong><u>HY Chang*</u></strong>. (2019) Structural analysis of chloroplast tail-anchored membrane protein recognition by ArsA1, The Plant Journal, 99:128-143.</span></li>
<li><span style="font-size:110%;"><strong><u>HY Chang*</u></strong>, TP Ko, YC Chang, KF Huang, CY Lin, HY Chou, CY Chiang, HJ Tsai. (2019) Crystal structure of the blue fluorescent protein with a Leu-Leu-Gly tri-peptide chromophore derived from the purple chromoprotein of Stichodactyla haddoni, Int. J. Biol. Macromol. 130: 675-684.</span></li>
<li><span style="font-size:110%;"><strong><u>HY Chang*</u></strong>, ST Lin, TP Ko, SM Wu, TH Lin, YC Chang, KF Huang, TM Lee. (2017) Enzymatic characterization and crystal structure analysis of Chlamydomonas reinhardtii dehydroascorbate reductase and their implications for oxidative stress, Plant Physiol. Biochem. 120: 144-155.</span></li>
<li><span style="font-size:110%;">M Maestre-Reyna, SM Wu, YC Chang, CC Chen, A Maestre-Reyna, AHJ Wang, <strong><u>HY Chang*</u></strong>. (2017) In search of tail-anchored protein machinery in plants: reevaluating the role of arsenite transporters, Scientific Report 7:46022 | DOI: 10.1038/srep46022.</span></li>
<li><span style="font-size:110%;"><strong><u>HY Chang</u></strong>, CC Chou, ML Wu, AHJ Wang. (2017) Expression, purification and enzymatic characterization of undecaprenyl pyrophosphate phosphatase from Vibrio vulnificus, Protein Expr. Purif. 133: 121-131.</span></li>
<li><span style="font-size:110%;">C von Ballmoos, I Smirnova, F Poiana, N Gonska, <strong><u>HY Chang</u></strong>, RB Gennis, P Brzezinski, P Ädelroth. (2017) Dynamics of the KB proton pathway in cytochrome ba3 from Thermus thermophiles, Isr. J. Chem. 57: 1-14. DOI: 10.1002/ijch.201600136.</span></li>
<li><span style="font-size:110%;">ST Lin, CW Chiou, YL Chu, YH, YF Tseng, YC Chen, HJ Chen, <strong><u>HY Chang</u></strong>, TM Lee. (2016) Enhanced ascorbate regeneration via dehydroascorbate reductase confers tolerance to photo-oxidative stress in Chlamydomonas reinhardtii. Plant Cell Physiol. 10: 2104-2121.</span></li>
<li><span style="font-size:110%;">A Mateja, M Paduch, <strong><u>HY Chang</u></strong>, A Szydlowska, AA Kossiakoff, RS Hegde, RJ Keenan. (2015) Structure of the Get3 targeting factor in complex with its membrane protein cargo. Science. 347: 1152-1155.</span></li>
<li><span style="font-size:110%;"><strong><u>HY Chang</u></strong>, CC Chou, MF Hsu, AHJ Wang. (2014) Proposed carrier lipid-binding site of undecaprenyl pyrophosphate phosphatase from Escherichia coli. J. Biol. Chem. 289: 18719-18735.</span></li>
<li><span style="font-size:110%;">I Smirnova, <strong><u>HY Chang</u></strong>, C von Ballmoos, P Ädelroth, RB Gennis, P Brzezinski. (2013) Single mutations that redirect internal proton transfer in the ba3 oxidase from Thermus thermophilus. Biochemistry 52: 7022-7030.</span></li>
<li><span style="font-size:110%;"><strong><u>HY Chang</u></strong>, SK Choi, AS Vakkasoglu, Y Chen, J Hemp, JA Fee, RB Gennis. (2012) Exploring the proton pump and exit pathway for pumped protons in cytochrome ba3 from Thermus thermophilus. Proc Natl Acad Sci U S A 109: 5259-5264.</span></li>
<li><span style="font-size:110%;"><strong><u>HY Chang</u></strong>, Y Ahn, LA Pace, MT Lin, YH Lin, RB Gennis. (2010) The diheme cytochrome c4 from Vibrio cholerae is a natural electron donor to the cbb3-type cytochrome c oxidase. Biochemistry 49: 7494-7503.</span></li>
<li><span style="font-size:110%;">I Smirnova, J Reimann, C von Ballmoos, <strong><u>HY Chang</u></strong>, RB Gennis, JA Fee, P Brzezinski, P Ädelroth. (2010) Functional role of Thr-312 and Thr-315 in the proton-transfer pathway in ba3 cytochrome c oxidase from Thermus thermophilus. Biochemistry 49: 7033-7049.</span></li>
<li><span style="font-size:110%;"><strong><u>HY Chang</u></strong>, J Hemp, Y Chen, JA Fee, RB Gennis. (2009) The cytochrome ba3 oxygen reductase from Thermus thermophilus uses a single input channel for proton delivery to the active site and for proton pumping. Proc Natl Acad Sci U S A 106: 16169-16173.</span></li>
<li><span style="font-size:110%;">TH Kuo, SC Chuang, <strong><u>SY Chang</u></strong>, PH Liang. (2006) Ligand specificities and structural requirements of two Tachypleus plasma lectins for bacterial trapping. Biochem. J. 393: 757-766.</span></li>
<li><span style="font-size:110%;">APC Chen, <strong><u>SY Chang</u></strong>, YC Lin, YS Sun, CT Chen, AHJ Wang, PH Liang. (2005) Substrate and product specificities for cis-type undecaprenyl pyrophosphate synthase. Biochem. J. 386: 169-176.</span></li>
<li><span style="font-size:110%;"><strong><u>SY Chang</u></strong>, TP Ko, APC Chen, AHJ Wang, PH Liang. (2004) Substrate binding mode and reaction mechanism of undecaprenyl pyrophosphate synthase deduced from crystallographic studies. Protein Sci. 13: 971-978.</span></li>
<li><span style="font-size:110%;"><strong><u>SY Chang</u></strong>, TP Ko, PH Liang, AHJ Wang. (2003) Catalytic mechanism revealed by the crystal structure of undecaprenyl pyrophosphate synthase in complex with sulfate, magnesium, and Triton. J. Biol. Chem. 278: 29298-29307.</span></li>
<li><span style="font-size:110%;"><strong><u>SY Chang</u></strong>, YK Chen, AHJ Wang, PH Liang. (2003) Identification of the active conformation and the importance of length of the flexible loop 72-83 in regulating the conformational change of undecaprenyl pyrophosphate synthase. Biochemistry. 42: 14452-14459.</span></li>
<li><span style="font-size:110%;">CE Atreya, EF Johnson, J Williamson, <strong><u>SY Chang</u></strong>, PH Liang, KS Anderson. (2003). Probing electrostatic channeling in protozoal bifunctional thymidylate synthase-dihydrofolate reductase using site-directed mutagenesis. J. Biol. Chem. 278: 28901- 28911.</span></li>
<li><span style="font-size:110%;"><strong><u>SY Chang</u></strong>, PC Tsai, CS Tseng, PH Liang. (2001) Refolding and characterization of a yeast dehydrodolichyl diphosphate synthase overexpressed in Escherichia coli. Prot. Exp. Puri. 23: 432-43.</span></li>
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