Biomedical Imaging
- Update Date:2025-01-15
- Units:Instrumentation Resource Center
[YangMing] Small animal optical imaging system: Biospace Lab PhotonIMAGER Optima

- Biospace Lab PhotonIMAGER Optima
- Instrument Location: East side of Experimental Animal Center, Yangming Campus
- Administrator: Prof. Lin, Ching-po
- Campus extension 67338
- Management unit: Yang-Ming Campus Small Animal Imaging Core Facility of Brain Science Research Center
- Office Location: Room 532, 5th Floor, Traditional Medicine Building A
- E-mail: nycu.saic@gmail.com
- Extension 65293
Optima has high-sensitivity imaging technology, which can go from general two-dimensional to three-dimensional imaging, and even has exclusive real-time dynamic imaging (real-time) and awake mode. Depending on the experimental design, researchers can use luminescence or fluorescent labeling technology to study research objects, such as genes, tumor cells, stem cells, antibodies, drugs, etc. It can be used in cancer research, new drug development, nanomaterial research, establishment of disease animal models, neurological function research, stem cell research, etc.

Luminescence imaging (BLI)

Fluorescence imaging (GFP)

Multi-probe imaging (BLI+GFP)
Absolute quantitative analysis and measurement of luminescence and fluorescence
Absolute quantitative analysis and measurement functions of luminescence and fluorescence
Intensity profile
With real-time dynamic imaging, pharmacokinetic analysis can be performed.

Simulating three-dimensional in vivo optical and computed tomography fusion images

Awake mode imaging

- The current opening hours are from 9 am to 5 pm, Monday to Friday (closed on holidays).
- Other sessions might be open and subject to further announcement based on equipment usage and the status of the technical staff.
Reservations
- NYCU and Taipei Veterans General Hospital users can make reservations four weeks before.
- Users from off-campus units can make reservations three weeks in advance.
- All billing and payment procedures must be completed before the experiment. Otherwise, all appointments for the month will be canceled.
- Reservations must be made at least seven days in advance, and the reservation time is in 1-hour increments.
- If you want to cancel the experiment, please log in to the reservation system seven days before the experiment to cancel it yourself; if you cancel 3-6 days before the experiment, half of the reservation time will be deducted; if you cancel within two days before the imaging day, the fee will be charged as usual.
- For new experiments and special requirements, please inform the operator of the experiment content one week before use.
- When the experiment exceeds the reservation time, if there are subsequent reservations, the use can be extended with the consent of the following unit for up to ten minutes. If other units have no subsequent reservation, the user can extend the session. If the extension exceeds twenty minutes, the session will be charged. Use less than one hour is counted as one hour.
- Please arrive on time according to the reserved time slot. Latecomers are not allowed to ask for a delay. Being late for 30 minutes will be considered a no-show, and the reservation will be canceled. The facility will suspend the users who are late or fail to show up repeatedly.
Specifications for Using the Instrument
- Due to limited computer storage space, Image files will be retained for up to 30 days.
- Currently, it is open for tissue and cell samples from general animals (bred on behalf of animal centers or in a temporary room approved by the animal center.) We do not provide services for infectious animals and samples.
- The facility only provides imaging services. We do not provide analysis services, contrast agents (CT contrast agent, luminescence, etc....), anesthetics (isoflurane only provides gas anesthesia machines), and consumables (needles, black cardboard, etc....).
- Gas anesthetics other than Isoflurane in gas anesthesia devices are prohibited.
- Users are responsible for any consequences of using Isoflurane anesthesia or administering contrast agents during animal imaging.
- The core facility is not responsible for keeping items deposited by users.
- Each full-time NYCU faculty member has two hours of free operation to facilitate the acquisition of preliminary research materials (including the teacher himself, who can only use the free operation quota of three teachers at most).
- Articles published in the name of NYCU and mentioned, thanks to the small animal imaging core facility of NYCU Brain Research Center, can get rewards. Each article will be deducted the usage fee equivalent to impact factor times hours (in rounds, less than one point is calculated by one point). Only one application can be made per article.
- Acknowledgments:
We thank the Molecular Imaging Facility Small Animal Instrument Name and Brain Research Center at National Yang Ming Chiao Tung University for the technical support.
Charging standards
- NYCU and Taipei Veterans General Hospital: NT$2,000/hour
- Other academic units: NT$3,000/hour
- Industry: NT$3,000/hour
- The usage fee does not include the cost of luminescence, contrast agent, gas anesthesia agent, and positron contrast agent.
- The user must prepare related consumables such as luminescence, contrast agent, and isoflurane. The Small Animal Imaging Core Facility will not provide these.
Application process
Payment method (please follow the purchase method and order)
By Cash
By Cash
- Fill out the User Consent Certificate in duplicate and ask the project host to sign or seal it.
- Bring the user consent certificate (in duplicate) to the cashier group to pay (stamp, take receipt).
- Bring the User Consent Certificate (in duplicate), payment receipt, and IACUC certificate to the Small Animal Imaging Core Facility to log in for hours.
By Check
- Fill out the User Consent Certificate in duplicate and ask the project host to sign or seal it.
- Bring the cheque and the user consent certificate (in duplicate) to the cashier team to pay (stamp, take the receipt).
- Bring the User Consent Certificate (in duplicate), payment receipt, and IACUC certificate to the Small Animal Imaging Core Facility to log in for hours.
On-Campus Loan Methods
- Fill in the user certificate in duplicate, ask the project host to sign or seal it, and fill in the school loan slip in the accounting requisition system.
(Pre-loan application and write-off → payee details → beneficiary code key in "000" → NYCU internal transfer). - Prepare documents of on-campus loan slip and user consent certificate to the Small animal imaging core facility Office - Stamped (the stamped certificate is the loan voucher).
- After completing item (2), hold the on-campus loan receipt and loan voucher (with small animal imaging core facility certificate after the office seal) to the Accounting Office to apply for the loan.
- After completing item (3), wait approximately three to four weeks for the cashier team to notify you to collect your receipt.
Please prepare the following documents: the original copy of the user certificate (in duplicate) and the cashier's seal. - Present receipt, User Consent Certificate (in duplicate) stamped by the Cashier team, and IACUC attestation to the Small animal imaging core facility to log in for hours.
Related Image(s):