- <![CDATA[[YangMing] ELISA Reader: TECAN Sunrise]]><![CDATA[<div class="ed_model05 clearfix">
<div class="ed_pic_right"><img alt="Enzyme immunoassay analyzer " src="/userfiles/ordch/images/20231016103227012.jpg" /></div>
<ul>
<li class="ed_txt"><strong>ELISA Reader</strong></li>
<li class="ed_txt"><strong>Brand Model: </strong>TECAN Sunrise ™</li>
<li class="ed_txt"><strong>Instrument Location: </strong>Yang-Ming Campus
<ul>
<li class="ed_txt"><strong>Room 465, 4th floor, Biomedical Engineering Building <span style="color:#8e44ad;">(G machine)</span></strong></li>
<li class="ed_txt"><strong>Room 701, 7th floor, Biomedical Building <span style="color:#8e44ad;">(H machine)</span></strong></li>
<li class="ed_txt"><strong>Room B02, B1 floor, Shou-ren Building <span style="color:#8e44ad;">(E machine)</span></strong></li>
</ul>
</li>
<li class="ed_txt"><strong>Term of use: Available anytime</strong>
<ul>
<li class="ed_txt">Free of charge for NYCU users. Off-campus users, please contact the center before use.</li>
</ul>
</li>
<li class="ed_txt"><strong>Contact center staff：</strong>
<ul>
<li class="ed_txt"><strong>Extension：</strong>62382</li>
<li class="ed_txt"><strong>Email：</strong><a href="mailto:ycirc@nycu.edu.tw" title="ycirc@nycu.edu.tw">ycirc@nycu.edu.tw</a></li>
</ul>
</li>
</ul>
</div>]]>Instrumentation Resource Center 2026-04-17Introduction<div class="ed_model01 clearfix">
<div class="ed_txt"><strong>Instrument specifications: </strong>Wavelength range from 400 to 700 nm</div>
</div>
<div class="ed_model05 clearfix">
<div class="ed_pic_right"><img alt="sunrise-od" src="/userfiles/ordch/images/20231016103551920.jpg" /></div>
<ul>
<li class="ed_txt">Wavelength selection mode: single wavelength, dual wavelength.</li>
<li class="ed_txt">Absorbance range: 0.000 to 4.000 OD</li>
<li class="ed_txt">Measurement speed: 6 seconds for single wavelength, 8 seconds for dual wavelength</li>
</ul>
</div>
<div class="ed_model01 clearfix">
<div class="ed_txt"><strong>This machine can perform the following experiments:</strong></div>
<ol>
<li class="ed_txt">Basic LEISA enzyme color reaction<br />
<img alt="Basic LEISA enzyme color reaction" src="/userfiles/ordch/images/20231016103844869.jpg" /></li>
<li class="ed_txt">Kinetic Assay <br />
<img alt="Kinetic Assay Enzyme Dynamic Experiment" src="/userfiles/ordch/images/20231016103919917.jpg" /></li>
<li class="ed_txt">Spectral Scan<br />
<img alt="Spectral Scan sample scan" src="/userfiles/ordch/images/20231016103953514.jpg" /></li>
<li class="ed_txt">Bradford Protein Assay for Protein Quantification (595nm)<br />
<img alt="Bradford Protein Assay Protein Quantification (595nm)" src="/userfiles/ordch/images/20231016104012384.jpg" /></li>
<li class="ed_txt">MTT Assay for Cell Viability (570nm)<br />
<img alt="MTT Assay Cell Viability (570nm)" src="/userfiles/ordch/images/20231016104027487.jpg" /></li>
<li class="ed_txt">LAL – Kinetic Assay for Endotoxin Test</li>
</ol>
</div>Software Applications<div class="ed_model01 clearfix">
<ol>
<li class="ed_txt">The interface is designed in a Genie style for guided operation.</li>
<li class="ed_txt">It is capable of performing qualitative, quantitative, or enzyme kinetic tests simultaneously.</li>
<li class="ed_txt">For each disk, it allows the setting of 6 different experiments along with their calculation parameters.</li>
<li class="ed_txt">A wide range of analysis parameters can be customized, including cut-off, concentration standard, extrapolation percentage, dilution factor, regression method, chart display method, automatic QC parameters, calculated original value, and comparison control value. It also allows for the conversion of the original value into a report.</li>
<li class="ed_txt">Cycle parameters, termination conditions, and settings for linear or quadratic slope calculations, as well as onset conditions for enzyme kinetic method tests, can be adjusted.</li>
<li class="ed_txt">The system is equipped with comprehensive built-in calculation formulas. Users can define constants and variables, perform calculations, and utilize statistical and logical operation functions.</li>
<li class="ed_txt">Reports are automatically generated and can be customized in format (matrix or tabular) and content, including standard linear graphs, averages, concentrations, negatives, positives, and cut-offs. They can also be organized based on specific results.</li>
<li class="ed_txt">The results of experiments can be exported in EXCEL or ASCII format, with customizable output information.</li>
</ol>
</div>Operation Manual<div class="ed_model17 clearfix ed_list">
<ul>
<li class="d-flex"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;">Operation Manual </span> </span>
<ul class="d-flex">
<li><a href="/userfiles/ordch/files/20231016104255782.pdf" rel="noreferrer noopener" target="_blank" title="Operation Manual (pdf) (Open a new window)"><img alt="Operation Manual " src="/userfiles/file/pdf.png" /> </a></li>
</ul>
</li>
</ul>
</div>Related Links<a rel="noopener noreferrer" href="https://lifesciences.tecan.com/products/microplate_readers/sunrise" target="_blank" title="TECAN sunrise(Open New Windows)"><img alt="TECAN sunrise" src="https://www.nycu.edu.tw/userfiles/ordch/images/20231016111223919.jpg"></a>https://www.nycu.edu.tw/ord/en/app/machine/image?module=machine&detailNo=1167366943485726720&init=YELISA Readerhttps://ord.edu.tw/ord/en/app/artwebsite/view?module=artwebsite&id=2023&serno=da69be39-dc4e-48d6-9168-7c96b2dbdb6eInstrument List of YM Campushttps://www.nycu.edu.tw/ord/en/app/machine/list?module=machine&id=2041Other Plate Readers
- <![CDATA[[YangMing] Multimode microplate readers: Thermo Varioskan LUX]]><![CDATA[<div class="ed_model05 clearfix">
<div class="ed_pic_right"><img alt="All-wavelength absorption light and fluorescence composite analysis system Varioskan LUX" src="/userfiles/ordch/images/20231017083217299.jpg" /></div>
<ul>
<li class="ed_txt"><strong>Spectral Scanning Multimode Reader</strong>
<ul>
<li class="ed_txt"><strong>Brand Model: </strong>Thermo Varioskan LUX</li>
</ul>
</li>
<li class="ed_txt"><strong>Instrument Location: </strong>Yang-Ming Campus
<ul>
<li class="ed_txt"><strong>Room B02, B1 floor, Shou-ren Building <span style="color:#8e44ad;">(M</span><span style="color:#9b59b6;">achine </span><span style="color:#8e44ad;">G)</span><span style="color:#e74c3c;">*</span></strong></li>
<li class="ed_txt"><strong>Room 615, 6F. Library, Information & Research Building <span style="color:#9b59b6;">(Machine K)</span></strong></li>
</ul>
</li>
<li class="ed_txt"><strong>Term of Use: Available anytime</strong>
<ul>
<li class="ed_txt">Free of charge for NYCU users. Off-campus users, please contact the center before use.</li>
</ul>
</li>
<li class="ed_txt"><strong>Contact center staff：</strong>
<ul>
<li class="ed_txt"><strong>Extension：</strong>62382</li>
<li class="ed_txt"><strong>Email：</strong><a href="mailto:ycirc@nycu.edu.tw" title="ycirc@nycu.edu.tw">ycirc@nycu.edu.tw</a></li>
</ul>
</li>
</ul>
</div>
<div class="ed_txt">
<div class="ed_txt"> </div>
</div>]]>Instrumentation Resource Center 2025-07-29Instrument introduction<div class="ed_model01 clearfix">
<div class="ed_txt">The Varioskan LUX is a composite analysis system that detects full-wavelength absorbance and fluorescence. It covers various detection wavelengths, including visible light, luminescence, and fluorescence. The system uses a Xenon flash lamp as its light source. It has a built-in micro dispenser and support analysis applications for 6 to 1536-well plates.<br />
<br />
<strong>Features:</strong></div>
<ol>
<li class="ed_txt">Wavelength range:
<ul>
<li class="ed_txt">Absorbance: 200nm to 1000nm; Path Length is 1nm.</li>
<li class="ed_txt">Fluorescence: excitation range from 200nm to 1000nm, emission range from 270nm to 840nm.
<ul>
<li class="ed_txt"><span style="color:#ff0000;">To effectively measure fluorescence, the wavelength between the fluorescence excitation and absorption should be at least 18nm if the excitation bandwidth is 5nm. </span>For instance, if the fluorescence excitation wavelength is currently set at 400nm, the absorption wavelength must be at least 418nm. </li>
<li class="ed_txt"><span style="color:#ff0000;">On the other hand, if you choose a 12nm excitation bandwidth, the wavelength between the fluorescence excitation and absorption should be at least 25nm. </span>In this case, assuming that the fluorescence excitation wavelength is set to 400nm, the absorption wavelength needs to be at least 425nm for effective measurement.</li>
</ul>
</li>
<li class="ed_txt">Luminescence: 270mm~840 nm, bandwidth 12 nm</li>
</ul>
</li>
<li class="ed_txt">Sample Type:
<ul>
<li class="ed_txt">Absorbance: 6 to 384-well plate.</li>
<li class="ed_txt">Fluorescence: 6 to 1536-well plate.</li>
<li class="ed_txt">Luminescence: 6 to 1536-well plate.</li>
</ul>
</li>
<li class="ed_txt">Detection Range:
<ul>
<li class="ed_txt">Absorbance: 0~4 Abs.</li>
<li class="ed_txt">Fluorescence: >6 decades, 384-well plate</li>
<li class="ed_txt">Luminescence: ≧7 decades.</li>
</ul>
</li>
<li class="ed_txt">It automatically corrects the light path with water or other solvents to calculate the equivalent correction value of a 1cm cuvette.</li>
<li class="ed_txt">Temperature control range: Ambient +4℃～45℃.</li>
<li class="ed_txt">Dispenser Module <strong>(ONLY Machine G<span style="color:#ff0000;">*</span>)</strong>
<ul>
<li class="ed_txt">Built-in two automatic dispensers, supporting 6 to 384-well plate analysis applications.</li>
<li class="ed_txt">The volume range must cover 2 ~ 5,000 μl, and the accuracy must be <0.2 μl.</li>
</ul>
</li>
<li class="ed_txt">Software
<ul>
<li class="ed_txt">With Endpoint, Kinetic, Spectra, Kinetic spectra, and Multipoint measurement modes.</li>
<li class="ed_txt">Data output supports Excel, PDF, XML, and TXT formats; instrument operation reports (Log files) can be exported to track instrument usage.</li>
</ul>
</li>
</ol>
</div>Usage Rules<div class="ed_model01 clearfix">
<ol>
<li class="ed_txt">This machine is available on demand.</li>
<li class="ed_txt"><span style="color:#ff0000;">After use, please make sure to check if the plate has been taken out and the holder has been returned to the machine. Also, remember to turn off the light source.</span></li>
<li class="ed_txt">If you're using the default parameters, please save a new file first to avoid overwriting.</li>
<li class="ed_txt">By using the shaking function, you can utilize the well volume up to 200μl.</li>
<li class="ed_txt">When using the dispenser, remember to prime and empty it, and keep the pipeline clean.</li>
</ol>
</div>Manuals and Instructional Videos<div class="ed_model17 clearfix ed_list">
<ul>
<li class="d-flex"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;">Operation Manual</span> </span>
<ul class="d-flex">
<li><a href="/userfiles/ordch/files/20231017084003112.pdf" rel="noreferrer noopener" target="_blank" title="Simple Operation Manual (pdf) (Open a new window)"><img alt="Simple Operation Manual" src="/userfiles/file/pdf.png" /> </a></li>
</ul>
</li>
<li class="d-flex"><span style="vertical-align: inherit;">User Manual</span>
<ul class="d-flex">
<li><a href="#" rel="noreferrer noopener" target="_blank" title="User Manual(開啟新視窗)"><img alt="User Manual" src="/userfiles/file/pdf.png" /> </a></li>
</ul>
</li>
<li class="d-flex"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;">Quick Start Guide</span></span>
<ul class="d-flex">
<li><a href="#" rel="noreferrer noopener" target="_blank" title="Quick Start Guide(Open New Windows)"><img alt="Quick Start Guide" src="/userfiles/file/pdf.png" /> </a></li>
</ul>
</li>
<li class="d-flex">Instruction Video Resources from Thermo Fisher Scientific
<ul class="d-flex">
<li><a href="https://www.thermofisher.com/tw/zt/home/life-science/lab-equipment/microplate-instruments/plate-readers/resources.html" rel="noreferrer noopener" target="_blank" title="Video Resources of Thermo Fisher(Open New Windows)"><img alt="Video Resources of Thermo Fisher" src="/userfiles/file/link.png" /> </a></li>
</ul>
</li>
<li class="d-flex">"Varioskan LUX Simple Operation" ( Play 12:06 )
<ul class="d-flex">
<li><a href="https://youtu.be/5ZjJN8Isqbg" rel="noreferrer noopener" target="_blank" title="Open New Windows"><img alt=""Varioskan LUX Simple Operation" ( Play 12:06 )" src="/userfiles/file/link.png" /> </a></li>
</ul>
</li>
<li class="d-flex">"Varioskan LUX Data Analysis" ( Play 20:49 )
<ul class="d-flex">
<li><a href="https://youtu.be/2CtUBRb4S9A" rel="noreferrer noopener" target="_blank" title="Open New Windows"><img alt=""Varioskan LUX Data Analysis" ( Play 20:49 )" src="/userfiles/file/link.png" /> </a></li>
</ul>
</li>
<li class="d-flex">"Varioskan LUX Dispenser" ( Playback 5:54 )
<ul class="d-flex">
<li><a href="https://youtu.be/Z6pASJgirB4" rel="noreferrer noopener" target="_blank" title="Open New Windows"><img alt=""Varioskan LUX Dispenser" ( Playback 5:54 )" src="/userfiles/file/link.png" /> </a></li>
</ul>
</li>
<li class="d-flex">"Varioskan LUX with uDrop Duo Plate "（Playback 45:33 ）
<ul class="d-flex">
<li><a href="https://youtu.be/IyprtIlIdBY?si=yrrwUb83ooykw-HB" rel="noreferrer noopener" target="_blank" title="Varioskan LUX with uDrop Duo Plate(Open New Windows)"><img alt="Varioskan LUX with uDrop Duo Plate" src="/userfiles/file/link.png" /> </a></li>
</ul>
</li>
</ul>
</div>Related Links<div class="ed_model01 clearfix">
<div class="ed_txt"><a href="https://www.thermofisher.com/tw/zt/home.html?CID=EBZ_BUS_SBU_R04_TW_cp0000_pjt0000_bid00000_0SE_gaw_pt_PUR_TFS_gene_aBrand_pc_hp_113020_0000000A1E1FE9C6" rel="noreferrer noopener" target="_blank" title="ThermoFisher (Open a new window)"><img alt="ThermoFisher" src="/userfiles/ordch/images/20231017084212978.png" /></a></div>
</div>https://www.nycu.edu.tw/ord/en/app/machine/image?module=machine&detailNo=1167364586639200256&init=YThermo Varioskan LUXhttps://ord.edu.tw/ord/en/app/artwebsite/view?module=artwebsite&id=2023&serno=da69be39-dc4e-48d6-9168-7c96b2dbdb6eInstrument List of YM Campushttps://www.nycu.edu.tw/ord/en/app/machine/list?module=machine&id=2041Other Plate Readers
- <![CDATA[[YangMing] Multimode microplate reader - TECAN 200/200Pro]]><![CDATA[<div class="ed_model05 clearfix">
<div class="ed_pic_right"><img alt="Multifunctional Micro Disc Analyzer TECAN 200/200Pro" src="/userfiles/ordch/images/20231016104820640.jpg" /></div>
<ul>
<li class="ed_txt"><strong>Multimode microplate reader</strong></li>
<li class="ed_txt"><strong>Brand / Model</strong>
<ul>
<li class="ed_txt">TECAN Infinite 200 PRO： Machine C, E, F </li>
<li class="ed_txt">TECAN Infinite 200：Machine B</li>
</ul>
</li>
<li class="ed_txt"><strong>Instrument Location: </strong>Yang-Ming Campus
<ul>
<li class="ed_txt"><strong>Room 465, 4F. Biomedical Engineering Building<span style="color:#8e44ad;"> (Machine E)</span> </strong></li>
<li class="ed_txt"><strong>Room 701, 7F. Biomedical Building <span style="color:#8e44ad;">(Machine B), (Machine F)</span><span style="color:#e74c3c;">*</span> </strong></li>
<li class="ed_txt"><strong>Room 615, 6F. Library, Information & Research Building <span style="color:#8e44ad;">(Machine C)</span></strong></li>
</ul>
</li>
<li class="ed_txt"><strong>Terms of Use: Available anytime</strong>
<ul>
<li class="ed_txt"><span style="color:#ff0000;">Only the F machine can be used for long-term experiments and must be reserved online through the <a href="https://ircbooking.nycu.edu.tw/IRC/irc_booking/index_en.aspx" target="_blank"><span style="color:#ff0000">"Instrument Reservation System</span></a>." </span></li>
<li class="ed_txt">Free of charge for NYCU users. Off-campus users, please contact the center before use.</li>
</ul>
</li>
<li class="ed_txt"><strong>Contact center staff：</strong>
<ul>
<li class="ed_txt"><strong>Extension：</strong>62382</li>
<li class="ed_txt"><strong>Email：</strong><a href="mailto:ycirc@nycu.edu.tw" title="ycirc@nycu.edu.tw">ycirc@nycu.edu.tw</a></li>
</ul>
</li>
</ul>
</div>]]>Instrumentation Resource Center 2025-07-28Introduction<div class="ed_model12 clearfix">
<div class="item01">
<p><strong>I.</strong></p>
<strong> Specifications</strong>
<ol>
<li>Detection modes include absorbance, absorbance full-wavelength scanning, fluorescence upper and lower detection, fluorescence full-wavelength scanning, time-lag fluorescence detection, luminescence, and dual-color luminescence detection modes.</li>
<li>A light source system supports all detection modes and uses a high-energy Xenon lamp xenon flash lamp. The lamp has a long life and does not need to be warmed up before use.</li>
<li>The number of flashes of the xenon bulb can be adjusted according to different detection modes.</li>
<li>During interpretation, the light source will be detected through the Reference Fiber, and the correctness of the penetrating light source and the stability of the bulb flicker will be confirmed.</li>
<li>Detector: Match the best detector according to different detection modes (up to 3 can be installed).</li>
<li>Multiple calibrations and interpretations can be performed. The absorbance and fluorescence detection can be performed simultaneously on the same microplate.</li>
<li><strong>Sample format: 6~384 microwell plate, plate height up to 23mm (microplate covered). You do not need to replace the Plate Adapter or Plate Holder when using different microplates.</strong></li>
<li>Wavelength screening: Continuous full-wavelength screening through a quadruple splitting system, no need to purchase additional filters.<br />
<img alt="Wavelength screening: Continuous full-wavelength screening through a quadruple light splitting system, no need to purchase additional filters." src="/userfiles/ordch/images/20231016105128695.jpg" /></li>
<li>The full-wavelength optical system is the Quad4 Monochromator system, which can effectively filter stray light up to 10-6 times. By rotating the Monochromator Mirror, the scanning speed is fast and accurate.</li>
<li>Shaking mode: Linear or Orbital Shaking, providing six levels of amplitude-frequency, which can be finely adjusted by 0.5 mm.</li>
<li>The device can be operated directly on the panel to move the microplate in and out.</li>
<li>Measurement speed: usually less than 20 seconds for a 96-well plate.</li>
<li>Built-in power supply.</li>
<li>The device uses voltage in the 120 - 240 volts range and has automatic induction.</li>
<li>The host has a USB 2.0 interface.</li>
<li>Temperature control: The device can maintain a temperature between room temperature +5 to 42℃.</li>
</ol>
</div>
<div class="item01">
<p><strong>II. </strong></p>
<strong>Absorbance measurement</strong>
<ol>
<li>Wavelength range: 230 to 1000 nm (UV/VIS/NIR)</li>
<li>Measurement range: 0 to 4 OD.</li>
<li>Detector module: UV Silicon photodiode</li>
<li>Accuracy: 0~2 OD. ≦ ±1 %</li>
<li>Wavelength accuracy: ≦ 315nm: ± 0.3 nm; >315 nm: ± 0.5 nm</li>
</ol>
</div>
<div class="item01">
<p><strong>III. </strong></p>
<strong>Fluorescence measurement</strong>
<ol>
<li>Measurement mode: upper and lower measurements support time-lag fluorescence measurement mode</li>
<li>Wavelength range: excitation range from 230 to 850nm; emission range from 280 to 850nm</li>
<li>Detection range: 0~60,000 RFU</li>
<li>PMT detector module: PMT, UV and red-sensitive</li>
<li>Fluorescence magnification Gain value setting: You can choose automatic adjustment (automatic analysis of the entire plate, analysis of specified hole positions, or enhanced dynamic range) or manual setting, and the range of 1~255 can be adjusted arbitrarily</li>
<li><strong>The interval between fluorescence excitation and absorption wavelengths must be ≧ 29nm.</strong> For example, if the fluorescence excitation wavelength is set to 400nm, the fluorescence absorbance wavelength must be ≧ 429nm for effective measurements.</li>
<li>Fluorescence sensitivity: The upper reading is 170 amol/well; the lower reading is 1.2 fmol/well</li>
<li>Wavelength accuracy: ≦315nm: ± 1 nm; >315 nm: ± 2 nm<br />
<img alt="fluorescence measurement chart" src="/userfiles/ordch/images/20231016105251052.jpg" /></li>
<li>During the upper reading experiment, the Z-position is automatically adjusted by 1μm to obtain the best fluorescence reading position. This adjustment is based on the difference in liquid level, and it helps to determine the optimal fluorescence signal and background value. A diagram represents the high position.</li>
</ol>
</div>
<div class="item01">
<p><strong>IV. </strong></p>
<strong>Luminescence measurement</strong>
<ol>
<li>Wavelength range: 380~600 nm</li>
<li>Linear range: >6 orders of magnitude</li>
<li>Detector module: Photon Counting System with low dark current PMT, a single photon counter that can accurately read luminescence readings with weak signals.</li>
<li>Glow Luminescence detection sensitivity: 225 amol ATP/well</li>
<li>Flash Luminescence detection sensitivity: 12 amol ATP/well</li>
<li>Integration Time/well: 1~20,000 ms<br />
<img alt="Cold light measurement" src="/userfiles/ordch/images/20231016105402092.jpg" /></li>
<li>The reading head's height can be adjusted automatically to the optimal Z-position reading height depending on the type of microplates used. This reduces the mutual interference caused by the self-illumination of luminescence samples and thereby improves the sensitivity of luminescence detection.</li>
<li>Built-in Filter Wheel supports dual-color luminescence detection mode in the following bands:
<ul>
<li>Green (510~540nm, Chroma-Glo, BRET2)</li>
<li>Magenta (610~700nm, Chroma-Glo, BRET2)</li>
<li>Blue1 (370~480nm, BRET)</li>
<li>Green1 (520~570, BRET)</li>
</ul>
</li>
</ol>
</div>
<div class="item01">
<p><strong>V. </strong></p>
<strong>Dispenser module</strong>
<ol>
<li>The module contains two dispensers and two sets of pipe holders, which can accurately dispense into 384 microwell plates</li>
<li>You can operate through the buttons on the panel which can directly perform Prime and Wash dispenser cleaning and maintenance.</li>
<li>Dispensing accuracy: <2% for 100 ul</li>
<li>Dead Volume: 100 ul, including pump back</li>
<li>Built-in pump-back recycling function can save reagents to reuse </li>
<li>Built-in dispenser and microplate sensors can confirm whether the microplate is correctly placed in the instrument to avoid dispensing errors</li>
<li>Dispense mode: You can choose the well mode (dispense each hole and interpret immediately) or the dispense mode (dispense the entire plate and then continue other actions, such as shaking, waiting, or reading)<br />
<img alt="Dispenser module" src="/userfiles/ordch/images/20231016105459174.jpg" /></li>
</ol>
</div>
</div>Software Applications<div class="ed_model01 clearfix">
<ol>
<li class="ed_txt">The software offers an intuitive, wizard-style guided operation interface that significantly improves work efficiency.</li>
<li class="ed_txt">It supports Endpoint, Kinetic, Multilabel, and more experiments.</li>
<li class="ed_txt">All experimental parameters and settings are conveniently displayed in the same window.</li>
<li class="ed_txt">The workflow-oriented software allows users to set the experimental process according to the analysis steps.</li>
<li class="ed_txt">Adding experimental steps is easy using the drop-down options, where users can define the microplate format, the number of readings, settling time, wavelength, and other experimental step settings.</li>
<li class="ed_txt">With the Multiassay function, one microwell plate can support various experiments and calculation parameters.</li>
<li class="ed_txt">The software has built-in enzyme kinetics formulas, and users can choose between Linear or Quadratic slope calculation methods and calculate the area under the curve they selected. The software also has built-in Lineweaver-Burk, Eadie-Hofstee, Hanes, and other drawing formulas for calculating Km and Vmax value formulas.</li>
<li class="ed_txt">One experimental method can be applied to several microplates without any changes.</li>
<li class="ed_txt">The software can read different combinations of filters and match different detection methods simultaneously.</li>
<li class="ed_txt">Users can set cycle parameters, reaction time differences, and specific conditions in the experimental cycle for enzyme kinetics.</li>
<li class="ed_txt">They can also set commands and reminders to be displayed during the experimental steps.</li>
<li class="ed_txt">After completing all measurements, the experimental data will be automatically saved in Excel format.</li>
</ol>
</div>Manuals and Instructional Videos<div class="ed_model17 clearfix ed_list">
<ul>
<li class="d-flex"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;">Magellan Software Manual </span> </span>
<ul class="d-flex">
<li><a href="/userfiles/ordch/files/20231016105841506.pdf" rel="noreferrer noopener" target="_blank" title="Magellan Software-Operation Manual(Open New Windows)"><img alt="Magellan Software-Operation Manual" src="/userfiles/file/pdf.png" /> </a></li>
</ul>
</li>
<li class="d-flex"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;">i-control Software Manual</span> </span>
<ul class="d-flex">
<li><a href="/userfiles/ordch/files/20231016105933479.pdf" rel="noreferrer noopener" target="_blank" title="i-control software-operation manual (pdf)(open new window)"><img alt="i-control software-operation manual" src="/userfiles/file/pdf.png" /> </a></li>
</ul>
</li>
<li class="d-flex"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;">i-control Software Manual (including dispensers)</span> </span>
<ul class="d-flex">
<li><a href="/userfiles/ordch/files/20231016110059297.pdf" title="i-control software-operation manual (including dispenser) (pdf)"><img alt="i-control software-operation manual" src="/userfiles/file/pdf.png" /> </a></li>
</ul>
</li>
<li class="d-flex"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;">i-control Software -Z position settings</span></span>
<ul class="d-flex">
<li><a href="/userfiles/ordch/files/20240507100902954.pdf" title="i-control-Z position settings(pdf)"><img alt="infinite 200 pro M_How to set z-position" src="/userfiles/file/pdf.png" /> </a></li>
</ul>
</li>
</ul>
</div>
<div class="ed_model01 clearfix">
<div class="ed_txt"><strong>Instruction Videos</strong></div>
<ul>
<li class="ed_txt">i-control software
<ul>
<li class="ed_txt"><a href="https://www.youtube.com/watch?v=21KzBpzwLMc&feature=youtu.be" rel="noreferrer noopener" target="_blank" title=""Software Introduction and Absorbed Light Measurement"(Open New Windows)">"Introduction and Absorbance Measurements"</a> ( 17:10 )</li>
<li class="ed_txt"><a href="https://www.youtube.com/watch?v=soUaVPomPSw&feature=youtu.be" rel="noreferrer noopener" target="_blank" title=""Fluorescence Reading"(Open New Windows)">"Fluorescence Measurements"</a> (10:18 )</li>
<li class="ed_txt"><a href="https://www.youtube.com/watch?v=hYEAoHeFi_M" rel="noreferrer noopener" target="_blank" title=""Cold Light Reading"(Open New Windows)">"Luminescence Measurements"</a> ( 5:33 )</li>
<li class="ed_txt"><a href="https://www.youtube.com/watch?v=bYZamdy6_Ik&feature=youtu.be" rel="noreferrer noopener" target="_blank" title=""Application Example Introduction"(Open New Windows)">"Application Demo, Injectors, etc."</a> ( 21:13 )</li>
</ul>
</li>
<li class="ed_txt">Magellan software
<ul>
<li class="ed_txt"><a href="https://youtu.be/N5PzBkspNxs" rel="noreferrer noopener" target="_blank" title=""Simple Measurements"(Open New Windows)">"Simple Measurements"</a> ( 8:25 )</li>
<li class="ed_txt"><a href="https://youtu.be/9GzRvqRtZEU" rel="noreferrer noopener" target="_blank" title=""Experimental Design (Including Standard curves and Graph)"(Open New Windows)">"Experimental Design (Including Standard curves and Graph)"</a> ( 17:47 )</li>
</ul>
</li>
</ul>
</div>Precautions<div class="ed_model01 clearfix">
<ol>
<li class="ed_txt">This machine is designed to be used at any time.</li>
<li class="ed_txt">Please confirm the<strong> instrument connection status </strong>in the lower left corner of the i-control software before use.<br />
<img alt="Please confirm the instrument connection status in the lower left corner of the i-control software before use" src="/userfiles/ordch/images/20231016110900892.jpg" /></li>
<li class="ed_txt">If the connection between the instrument and the software is lost, follow these steps to reconnect:
<ol>
<li class="ed_txt">Firstly, close the i-control software.</li>
<li class="ed_txt">Secondly, turn off the machine from the rear and wait for 30 seconds.
<ol>
<li class="ed_txt"><img alt="Turn off the power from the rear of the machine and wait 30 seconds" src="/userfiles/ordch/images/20231016110923908.jpg" /></li>
</ol>
</li>
<li class="ed_txt">Turn on the machine.</li>
<li class="ed_txt">After restarting the power, wait until the triangular green light on the machine panel is constantly on. Then, open the i-control software to reconnect.</li>
<li class="ed_txt"><img alt="After turning the power back on, wait until the triangular green light on the machine panel is always on and does not flash, open the i-contorl software and reconnect" src="/userfiles/ordch/images/20231016110947108.jpg" /></li>
</ol>
</li>
<li class="ed_txt">Open i-control software and reconnect steps:
<ol>
<li class="ed_txt">Click Instrument > Connect<br />
<img alt="Click Instrument > Connect" src="/userfiles/ordch/images/20231016111020915.jpg" /></li>
<li class="ed_txt">Choose<strong> the instrument name</strong> on the opened window and click "OK."<br />
<img alt="After clicking the machine name in the newly opened window, click OK below" src="/userfiles/ordch/images/20231016111034581.jpg" /></li>
<li class="ed_txt">The instrument reconnects successfully if the serial number is shown.<br />
<img alt="Reconnected successfully" src="/userfiles/ordch/images/20231016111104540.jpg" /></li>
</ol>
</li>
<li class="ed_txt">Please keep the i-Control software open!</li>
</ol>
</div>Related Links<div class="ed_model01 clearfix">
<div class="ed_txt"><a href="https://lifesciences.tecan.com/plate_readers/infinite_200_pro" rel="noreferrer noopener" target="_blank" title="TECAN(Open in new window)"><img alt="TECANＮ" src="/userfiles/ordch/images/20231016111223919.jpg" /></a><br />
<a href="https://www.tecan.com/scaling-3d-spheroid-and-organoid-cell-experiments" rel="noreferrer noopener" target="_blank" title="Research Application Reference Materials(Open New Window)">Research Application References</a></div>
</div>https://www.nycu.edu.tw/ord/en/app/machine/image?module=machine&detailNo=1168800035811364864&init=YMultifunctional Micro Disc Analyzer TECAN 200/200Prohttps://ord.edu.tw/ord/en/app/artwebsite/view?module=artwebsite&id=2023&serno=da69be39-dc4e-48d6-9168-7c96b2dbdb6eInstrument List of YM Campushttps://www.nycu.edu.tw/ord/en/app/machine/list?module=machine&id=2041Other Plate Readers
- <![CDATA[[YangMing] Multifunctional Microplate Reader: TECAN SPARK]]><![CDATA[<div class="ed_model05 clearfix">
<div class="ed_pic_right"><img alt="Multifunctional Micro Disc Analyzer TECAN SPARK" src="/userfiles/ordch/images/20231016111748134.jpg" /></div>
<ul>
<li class="ed_txt"><strong>Multimode microplate readers</strong></li>
<li class="ed_txt"><strong>Brand Model: </strong>TECAN SPARK</li>
<li class="ed_txt"><strong>Instrument Location: </strong>Yang-Ming Campus
<ul>
<li class="ed_txt"><strong>Room 465, 4th floor, Biomedical Engineering Building <span style="color:#9b59b6;">(H machine)</span> </strong></li>
<li class="ed_txt"><strong>Room B02, B1 floor, Shou-ren Building <span style="color:#9b59b6;">(I machine)</span> </strong></li>
<li class="ed_txt"><strong>Room 701, 7th floor, Biomedical Building <span style="color:#9b59b6;">(J machine)</span></strong></li>
</ul>
</li>
<li class="ed_txt"><strong>Term of Use: Available anytime </strong>
<ul>
<li class="ed_txt"><span style="color:#e74c3c;">Machine J of the Biomedical Building is open for weekend long-term experiments. Please make online reservations through the "<a href="https://ircbooking.nycu.edu.tw/IRC/irc_booking/index_en.aspx" rel="noreferrer noopener" target="_blank" title="Instrument Reservation System(Open New Windows)">Instrument Reservation System</a>."</span></li>
<li class="ed_txt">Free of charge for NYCU users. Off-campus users, please contact the center before use.</li>
</ul>
</li>
<li class="ed_txt"><strong>Contact center staff：</strong>
<ul>
<li class="ed_txt"><strong>Extension：</strong>62382</li>
<li class="ed_txt"><strong>Email：</strong><a href="mailto:ycirc@nycu.edu.tw" title="ycirc@nycu.edu.tw">ycirc@nycu.edu.tw</a></li>
</ul>
</li>
</ul>
</div>]]>Instrumentation Resource Center 2025-01-15Introduction<div class="ed_model12 clearfix">
<div class="item01">
<p><strong>I.</strong></p>
<strong> Instrument Specifications</strong>
<ol>
<li>The system utilizes a high-energy Xenon flash lamp as its light source. This lamp has a notable long lifespan and requires no pre-use warming.</li>
<li>The flash frequency of the Xenon bulb can be tailored to suit various detection modes.</li>
<li>Compatible with up to three detectors, the system allows the optimal detector to be chosen based on the detection mode.</li>
<li>Accommodates sample formats ranging from 6 to 384 microwell plates without changing the Plate Adapter or Plate Holder for different plate sizes.</li>
<li>Offers continuous full-wavelength screening via a quadruple splitting system, eliminating the need for additional filters.</li>
<li>Features the Quad4 Monochromator optical system for effective stray light filtration, up to a factor of 10<sup>-6</sup>, with rapid and precise scanning facilitated by rotating the Monochromator Mirror.</li>
<li>It provides three oscillation modes: linear, orbital, or double orbital shaking, with 11 amplitude frequency levels (330 rpm to 1440 rpm) adjustable by 0.5 mm increments.</li>
<li>Allows for direct operation on the panel, including microplate insertion and removal, without requiring a computer.</li>
<li>Delivers rapid measurement speeds: less than 13 seconds for a 96-well plate and less than 30 seconds for a 384-well plate.</li>
<li>Includes a built-in power supply.</li>
<li>Automatically adapts to voltages between 120 and 240 volts.</li>
<li>Equipped with a USB 2.0 interface for connectivity.</li>
<li>Features a temperature control range from room temperature +4 to 42℃±0.5℃.</li>
<li>Designed as a fully modular system, it allows for significant expansion flexibility.</li>
</ol>
</div>
<div class="item01">
<p><strong>II.</strong></p>
<strong> Absorbance Measurement</strong>
<ol>
<li>Independent optical system, dedicated high-speed Monochromator and detector</li>
<li>Wavelength range: 200 – 1000 nm (UV/VIS/NIR)</li>
<li>Measurement range: 0 – 4 OD.</li>
<li>Detector module: UV Silicon photodiode</li>
<li>Detection accuracy: @260 <0.5 %</li>
<li>Detection precision: @260 <0.2 %</li>
<li>Wavelength accuracy: ≦0.8 nm</li>
<li>Wavelength reproducibility: ≦0.5 nm</li>
<li>200-1000nm spectrum scanning speed: ≦5 sec</li>
</ol>
</div>
<div class="item01">
<p>III.</p>
<strong> Full Wavelength Fluorescence Measurement</strong>
<ol>
<li>Measurement mode: Supports upper and lower readings and time-lag fluorescence measurement mode.</li>
<li>Wavelength range: Excitation range from 230-900nm, while emission range from 280-900nm.</li>
<li>Adjustable Bandwidth: from 5 – 50nm, with options for 5, 7.5, 10, 15, 20, 25, 30, or 50nm.</li>
<li>Measurement range: from 0 to 60,000 RFU.</li>
<li>PMT detector module: Includes independent PMT, UV, and red-sensitive modules.</li>
<li>Fluorescence magnification Gain value setting: You can choose automatic adjustment (automatic analysis of the entire plate, analysis of specified hole positions, or enhanced dynamic range) or manual setting, and the range of 0~255 can be adjusted arbitrarily.</li>
<li>Fluorescence sensitivity: The upper reading is 20amol/well, while the lower reading is 0.2 fmol/well.</li>
<li>Time-lag fluorescence measurement sensitivity: 0.7amol/well.</li>
<li>Wavelength accuracy: ± 0.5 nm for ≦315nm and >315 nm.</li>
<li>During the upper reading experiment, the Z-position is automatically adjusted by 1μm to obtain the best fluorescence reading position. This adjustment is based on the difference in liquid level and helps determine the optimal fluorescence signal and background value. A diagram represents the high position.</li>
</ol>
</div>
<div class="item01">
<p><strong>IV. </strong></p>
<strong>Luminescence measurement</strong>
<ol>
<li>Wavelength range: 300-700 nm</li>
<li>Detector module: Independent Photon Counting System with low dark current PMT, single photon counter, which can accurately detect luminescence readings with weak signals.</li>
<li>Built-in Attenuation filter, effective detection dynamic range up to 109</li>
<li>Glow Luminescence detection sensitivity: 225 amol ATP/well,</li>
<li>Flash Luminescence detection sensitivity: 12 amol ATP/well</li>
<li>Integration Time/well: 1~20000 ms</li>
<li>The reading head's height can be adjusted automatically to the optimal Z-position reading height depending on the type of microplates used. This reduces the mutual interference caused by the self-illumination of luminescence samples, thereby improving luminescence detection sensitivity.</li>
<li>Cross Talk: 0.01%</li>
<li>Built-in long pass and short pass filters, supporting 19-band multi-color luminescence detection mode</li>
</ol>
</div>
</div>Software Applications<div class="ed_model01 clearfix">
<ol>
<li class="ed_txt">The software offers an intuitive, wizard-style guided operation interface that significantly improves work efficiency.</li>
<li class="ed_txt">It supports Endpoint, Kinetic, Multilabel, and more experiments.</li>
<li class="ed_txt">All experimental parameters and settings are conveniently displayed in the same window.</li>
<li class="ed_txt">The workflow-oriented software allows users to set the experimental process according to the analysis steps.</li>
<li class="ed_txt">Adding experimental steps is easy using the drop-down options, where users can define the microplate format, the number of readings, settling time, wavelength, and other experimental step settings.</li>
<li class="ed_txt">With the Multiassay function, one microwell plate can support various experiments and calculation parameters.</li>
<li class="ed_txt">The software has built-in enzyme kinetics formulas, and users can choose between Linear or Quadratic slope calculation methods and calculate the area under the curve they selected. The software also has built-in Lineweaver-Burk, Eadie-Hofstee, Hanes, and other drawing formulas for calculating Km and Vmax value formulas.</li>
<li class="ed_txt">One experimental method can be applied to several microplates without any changes.</li>
<li class="ed_txt">The software can read different combinations of filters and match different detection methods simultaneously.</li>
<li class="ed_txt">Users can set cycle parameters, reaction time differences, and specific conditions in the experimental cycle for enzyme kinetics.</li>
<li class="ed_txt">They can also set commands and reminders to be displayed during the experimental steps.</li>
<li class="ed_txt">After completing all measurements, the experimental data will be automatically saved in Excel format.</li>
</ol>
</div>Manuals and Instructional Videos<div class="ed_model17 clearfix ed_list">
<ul>
<li class="d-flex"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;">SPARK control Software-Chinese Simple Operation Manual</span> </span>
<ul class="d-flex">
<li><a href="/userfiles/ordch/files/20231016112417843.pdf" rel="noreferrer noopener" target="_blank" title="Dashboard Software-Chinese Simple Operation Manual (pdf) (Open a new window)"><img alt="Dashboard Software-Chinese Simple Operation Manual" src="/userfiles/file/pdf.png" /> </a></li>
</ul>
</li>
<li class="d-flex"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;">SPARK control -Z-position settings </span></span>
<ul class="d-flex">
<li><a href="/userfiles/ordch/files/20240507100619611.pdf" rel="noreferrer noopener" target="_blank" title="SPARK_How to set Z position(Open a new window)"><img alt="SPARK_How to set Z position" src="/userfiles/file/pdf.png" /> </a></li>
</ul>
</li>
<li class="d-flex"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;">Magellan Software - Chinese Simple Operation Manual</span> </span>
<ul class="d-flex">
<li><a href="/userfiles/ordch/files/20231016112501601.pdf" rel="noreferrer noopener" target="_blank" title="Magellan Software - Chinese Simple Operation Manual (pdf) (Open a new window)"><img alt="Magellan Software - Chinese Simple Operation Manual" src="/userfiles/file/pdf.png" /></a></li>
</ul>
</li>
<li class="d-flex">"Absorbance Measurement" (12:50)
<ul class="d-flex">
<li><a href="https://youtu.be/Uh2cS7PvDDo" rel="noreferrer noopener" target="_blank" title="Open New Windows"><img alt=""Absorbed Light Measurement" ( Play 12:50 )" src="/userfiles/file/link.png" /></a></li>
</ul>
</li>
<li class="d-flex">"Fluorescence Measurement" (17:09)
<ul class="d-flex">
<li><a href="https://youtu.be/z5ilvoqfwOg" rel="noreferrer noopener" target="_blank" title="Open New Windows"><img alt=""Fluorescence Reading" ( Playback 17:09 )" src="/userfiles/file/link.png" /></a></li>
</ul>
</li>
<li class="d-flex">"Luminescence reading and Kinetic" (13:54)
<ul class="d-flex">
<li><a href="https://youtu.be/-Kl7L3nl5og" rel="noreferrer noopener" target="_blank" title="Open New Windows"><img alt=""Cold light reading and Kinetic" ( Playback 13:54 )" src="/userfiles/file/link.png" /></a></li>
</ul>
</li>
</ul>
</div>Related Links<div class="ed_model01 clearfix">
<div class="ed_txt"><a href="https://lifesciences.tecan.com/plate_readers/infinite_200_pro" rel="noreferrer noopener" target="_blank" title="TECAN(Open in new window)"><img alt="TECANＮ" src="/userfiles/ordch/images/20231016111223919.jpg" /></a><br />
<a href="https://www.tecan.com/scaling-3d-spheroid-and-organoid-cell-experiments" rel="noreferrer noopener" target="_blank" title="Research Application Reference Materials(Open New Window)">Research Application References</a></div>
</div>https://www.nycu.edu.tw/ord/en/app/machine/image?module=machine&detailNo=1167366378080964608&init=YTECAN SPARKhttps://ord.edu.tw/ord/en/app/artwebsite/view?module=artwebsite&id=2023&serno=da69be39-dc4e-48d6-9168-7c96b2dbdb6eInstrument List of YM Campushttps://www.nycu.edu.tw/ord/en/app/machine/list?module=machine&id=2041Other Plate Readers