- <![CDATA[[YangMing] Freeze Dryer]]><![CDATA[<div class="ed_model05 clearfix">
<div class="ed_pic_right"><img alt="Freeze Dryer" src="/userfiles/ordch/images/20240408143831378.jpg" /></div>
<ul>
<li class="ed_txt"><strong>Freeze Dryer</strong></li>
<li class="ed_txt"><strong>Brand/Model:</strong>
<ul>
<li class="ed_txt"><span style="color:#3498db;">VirTis BTP-9EGE0X</span>: C machine </li>
<li class="ed_txt"><span style="color:#3498db;">Labconco 701811010</span>: D machine </li>
<li class="ed_txt"><span style="color:#3498db;">CryoDry CD8</span>: E machine </li>
</ul>
</li>
<li class="ed_txt"><strong>Instrument Location:</strong> Yang-Ming Campus
<ul>
<li class="ed_txt"><strong>Room 465-2, 4th floor, Biomedical Engineering Building </strong><span style="color:#8e44ad;">(D), (E)</span><strong> </strong></li>
<li class="ed_txt"><strong>Room 710, 7th floor, Biomedical Building </strong><span style="color:#8e44ad;">(C)</span></li>
</ul>
</li>
<li class="ed_txt"><strong>Term of use: </strong>
<ul>
<li class="ed_txt">Free of charge for NYCU users. Off-campus users, please contact the center before use.</li>
</ul>
</li>
<li class="ed_txt"><strong>Reservation >>> </strong><span style="color:#e74c3c;">Please see the reservation rules for details. For first time users, please contact the center staff.</span></li>
<li class="ed_txt"><strong>Contact Us: </strong>
<ul>
<li class="ed_txt">Extension: 62382</li>
<li class="ed_txt">E-mail: <a href="mailto:ycirc@nycu.edu.tw" title="ycirc@nycu.edu.tw">ycirc@nycu.edu.tw</a></li>
</ul>
</li>
</ul>
</div>]]>Instrumentation Resource Center 2025-01-15Introduction<div class="ed_model12 clearfix">
<div class="item01">
<p>1. </p>
The principle is to use the sublimation process. Freeze drying is a process whereby water or other solvent is removed from frozen material by converting the frozen water directly into vapor without the intermediate formation of liquid water. When the sample is freeze-dried, the size, shape, and composition are unchanged, and the cell nutrients and activity in the original sample can also be retained. <br />
<img alt="Operating Procedure" src="/userfiles/ordch/images/20231018131445076.jpg" style="background-color: var(--bs-body-bg); color: var(--bs-body-color); font-family: var(--bs-body-font-family); font-size: var(--bs-body-font-size); font-weight: var(--bs-body-font-weight); text-align: var(--bs-body-text-align);" />
<div class="ed_model08 clearfix">
<div class="ed_pic_full">The Continuous Series of Process:</div>
<ol>
<li class="ed_pic_full">Pre-freezing: freeze to below the freezing point</li>
<li class="ed_pic_full">Primary drying (sublimation reaction): maintain a low-pressure vacuum condition</li>
<li class="ed_pic_full">Secondary drying (heat drying): warm to remove residual moisture</li>
</ol>
</div>
</div>
<div class="item01">
<p>2. </p>
It can be used for long-term storage of microbial cultures, enzymes, blood, specimens, medicines, and space food. Solid products can also be chemically analyzed because they are more stable than liquids.</div>
<div class="item01">
<p> </p>
</div>
</div>Application<div class="ed_model01 clearfix">
<ul>
<li class="ed_txt">Pharmaceutical:
<ul>
<li class="ed_txt">Drug screening</li>
<li class="ed_txt">Long-term preservation</li>
<li class="ed_txt">Shelf life</li>
</ul>
</li>
<li class="ed_txt">Food department:
<ul>
<li class="ed_txt">Analytical additives</li>
<li class="ed_txt">Preservative</li>
<li class="ed_txt">Vegetable and fruit genetic engineering</li>
</ul>
</li>
<li class="ed_txt">Medicine:
<ul>
<li class="ed_txt">Burn dressing</li>
<li class="ed_txt">Analysis organization and preservation</li>
</ul>
</li>
<li class="ed_txt">Zoology:
<ul>
<li class="ed_txt">Identify stress, fertility, nutrition</li>
<li class="ed_txt">Concentrated sedatives for ease of dosing</li>
</ul>
</li>
<li class="ed_txt">Clinical:
<ul>
<li class="ed_txt">Analysis of blood (plasma) and urine samples for metabolism studies</li>
<li class="ed_txt">Blood cells, cord blood</li>
<li class="ed_txt">Preparation of sputum samples</li>
</ul>
</li>
<li class="ed_txt">Environmental:
<ul>
<li class="ed_txt">Analysis of water and soil samples for long-term storage</li>
<li class="ed_txt">Detect air pollution</li>
</ul>
</li>
<li class="ed_txt">Genetics:
<ul>
<li class="ed_txt">Replication and abnormal analysis in animals and humans</li>
<li class="ed_txt">DNA sequencing</li>
</ul>
</li>
<li class="ed_txt">Proteomics Proteomics:
<ul>
<li class="ed_txt">Protects heat-sensitive proteins</li>
</ul>
</li>
</ul>
</div>Usage and Reservation Rules<div class="ed_model01 clearfix">
<ol>
<li class="ed_txt">There are currently three freeze dryers and the opening hours are as follows:
<ul>
<li class="ed_txt">
<table border="1" cellpadding="1" cellspacing="1" class="ed_table caption-top">
<caption>Opening Hours and Rules</caption>
<tbody>
<tr>
<td>Location</td>
<td style="text-align: center;">Model </td>
<td>Outer / Inner slot</td>
<td>Scheduled on/off time</td>
</tr>
<tr>
<td colspan="1" rowspan="2">Room 465-2,<br />
4F. Biomedical Engineering Building</td>
<td style="text-align: center;">D</td>
<td>Available with outer slots only, which can be placed at any time</td>
<td>Every Thursday 8:30 am (OFF) - 11:30 am (ON)</td>
</tr>
<tr>
<td style="text-align: center;">E</td>
<td>Only available for<span style="color:#ff0000;"> inner slot*</span> use, required <span style="color:#ff0000;">online reservation</span> before use</td>
<td>Self-operation after reservation. <br />
For first-time users, please contact our staff.</td>
</tr>
<tr>
<td>Room 710,<br />
7F. Biomedical Building</td>
<td style="text-align: center;">C</td>
<td>
<ul>
<li>The outer slot is available for direct use</li>
<li><span style="color:#ff0000;">The inner slot*</span> requires a reservation on the log book and is available with a scheduled on/off time.</li>
<li><span style="color:#ff0000;">Each time the inner slot is reserved, the maximum capacity is 1/2 of the tank volume. Please use the outer slots if the capacity is less than 1/4, and the scheduled switch time must be matched.</span></li>
</ul>
</td>
<td>Every Monday and Thursday, 8:30 am (OFF) - 15:30 pm (ON)</td>
</tr>
</tbody>
</table>
</li>
</ul>
</li>
<li class="ed_txt">If no one is using it, users can switch it on and off by themselves. If you are unfamiliar with the switching process, contact the central office.</li>
</ol>
<div class="ed_txt"><span style="font-size:110%;">【Note】</span></div>
<ol>
<li class="ed_txt">Freeze-dry bottles can be brought by yourself or borrowed from the center in advance.</li>
<li class="ed_txt">Make sure that the freeze-dry bottle you prepare can be tightly sealed with the valve of the freeze-dryer and that there is no air leakage.</li>
<li class="ed_txt"><strong>The freeze-dry bottle is expensive, please use it carefully. <span style="color:#ff0000;">You must compensate for a new freeze-dried bottle (brand only VirTis or Labconco) if damaged.</span></strong></li>
<li class="ed_txt"><span style="color:#ff0000;"><em>It is strictly forbidden to use【Isotope】and organic solvents.</em></span></li>
</ol>
</div>References<div class="ed_model17 clearfix ed_list">
<ul>
<li class="d-flex">C Machine: Labconco Freeze Dryer User's Manual
<ul class="d-flex">
<li><a href="/userfiles/ordch/files/20231018131956696.pdf" rel="noreferrer noopener" target="_blank" title="Labconco Freeze Dryer User's Manual(開啟新視窗)"><img alt="Labconco Freeze Dryer User's Manual" src="/userfiles/file/pdf.png" /> </a></li>
</ul>
</li>
<li class="d-flex">D Machine: VirTis Freeze Dryer Manual
<ul class="d-flex">
<li><a href="/userfiles/ordch/files/20231018131943114.pdf" rel="noreferrer noopener" target="_blank" title="VirTis Benchtop 2K Manual(pdf)(開啟新視窗)"><img alt="VirTis Benchtop 2K Manual" src="/userfiles/file/pdf.png" /> </a></li>
</ul>
</li>
<li class="d-flex">E Machine: CryoDry CD8 Manual (CH)
<ul class="d-flex">
<li> </li>
<li> </li>
<li><a href="/userfiles/ordch/files/20240219154434946.pdf" target="_blank" title="CryoDry CD8 Manual (CH)(開啟新視窗)"><img alt="CryoDry CD8 Manual (CH)" src="/userfiles/file/pdf.png" /> </a></li>
</ul>
</li>
</ul>
</div>Related Links<div class="ed_model01 clearfix">
<div class="ed_txt"><img alt="LABCONCO" src="/userfiles/ordch/images/20231018132049274.png" /><br />
<a href="https://scientificproducts.com/products/sp-virtis-advantage-pro-freeze-dryer/" rel="noreferrer noopener" target="_blank" title="SP Scientific VirTis rebrands as ATS(Open New Windows)"><img alt="SP Scientific VirTis rebrands as ATS" src="/userfiles/ordch/images/20231018132156057.png" /></a>ATS Scientific VirTis<br />
<a href="https://cryodry.biz/product/cryodry-cd8/" target="_blank" title="CryoDry offcial site(Open New Windows)"><img alt="CryoDry offcial site" src="/userfiles/ordch/images/20240219154736564.jpg" /></a></div>
</div>https://www.nycu.edu.tw/ord/en/app/machine/image?module=machine&detailNo=1226783809215664128&init=YFreeze Dryerhttps://www.nycu.edu.tw/ord/en/app/artwebsite/view?module=artwebsite&id=2023&serno=da69be39-dc4e-48d6-9168-7c96b2dbdb6eInstrument List of YM Campushttps://www.nycu.edu.tw/ord/en/app/machine/list?module=machine&id=2060Concentrator and Dryer
- <![CDATA[[YM Campus] Vacuum Concentrator System: SpeedVac]]><![CDATA[<div class="ed_model05 clearfix">
<div class="ed_pic_right"><img alt="High-efficiency vacuum concentration centrifuge" src="/userfiles/ordch/images/20231018132503966.png" /></div>
<ul>
<li class="ed_txt"><strong>Vacuum Concentrator System</strong>
<ul>
<li class="ed_txt">Brand/Model: Thermo SPD121P SpeedVac* Kits</li>
</ul>
</li>
<li class="ed_txt"><strong>Instrument Location: </strong>Yang-Ming Campus
<ul>
<li class="ed_txt"><strong>Room 438-1, Biomedical Engineering Building</strong></li>
</ul>
</li>
<li class="ed_txt"><strong>Term of Use: </strong>
<ul>
<li class="ed_txt"><strong>Contact the center's technical staff to explain the dos and don'ts to be qualified to make reservations.</strong></li>
<li class="ed_txt">Free of charge for NYCU users. Off-campus users, please contact the center before using it.</li>
</ul>
</li>
<li class="ed_txt"><strong>Reservation >>> <a href="https://ircbooking.nycu.edu.tw/IRC/irc_booking/index_en.aspx" rel="noreferrer noopener" target="_blank" title="Instrument Reservation System(Open New Windows)">Instrument Reservation System</a></strong></li>
<li class="ed_txt"><strong>Contact center staff：</strong>
<ul>
<li class="ed_txt"><strong>Extension：</strong>62382</li>
<li class="ed_txt"><strong>Email：</strong><a href="mailto:ycirc@nycu.edu.tw" rel="noreferrer noopener" target="_blank" title="ycirc@nycu.edu.tw(Open New Windows)">ycirc@nycu.edu.tw</a></li>
</ul>
</li>
</ul>
</div>]]>Instrumentation Resource Center 2025-01-15Introduction<div class="ed_model01 clearfix">
<div class="ed_txt"><strong>The Principle of Concentrating centrifuge </strong><br />
Concentration centrifugation is a process that uses heat energy to evaporate the liquid solvent and concentrate precious samples. When the solvent evaporates, the remaining crystals are the substances originally dissolved in the solvent, such as purified DNA, RNA, or proteins.<br />
<br />
To evaporate solvents with high boiling points, low pressure can be used to reduce the boiling point. For example, water has a boiling point of 100°C at atmospheric pressure (1.0 bar), but at low pressure (0.48 bar), the boiling point drops to about 80°C. When using high-efficiency vacuum pumps to reach extremely low pressure (<<< 0.001 bar), water will evaporate quickly at room temperature.<br />
<br />
The high vacuum environment not only improves efficiency but also eliminates oxygen and other free radicals in the air, preventing protein oxidation or denaturation during the concentration process. To prevent sample loss due to sudden boiling under high vacuum conditions, centrifugal force can effectively push the sample to the bottom of the sample bottle. Additionally, a good rotor design can maintain the maximum surface area of the liquid by tilting it at a 45° angle with the centrifugal axis.<br />
<br />
When the solvent contains corrosive or harmful substances, a condensation system is required between the sample volatilization tank and the vacuum pump. This system helps to collect the condensed volatilized gas at a low temperature, reducing health and safety risks for users and prolonging the life of the vacuum pump.<br />
<br />
<strong>Features</strong>: <br />
The concentrator centrifuge includes the host (SPD121P), condensate tank, and vacuum pump.</div>
<ol>
<li class="ed_txt"><strong>The SPD series host</strong> is designed to concentrate biological and non-biological samples. It features TEFLON industrial-grade anti-corrosion coatings and precision rotors for different sample containers.</li>
<li class="ed_txt"><strong>The condensate tank, RVT series</strong>, provides a low-temperature waste liquid recycle function, ensuring that the vacuum pump is not corroded by organic substances. It uses Germany's Danfoss compressor, has a temperature as low as -50°C, and uses an environmentally friendly refrigerant.</li>
<li class="ed_txt"><strong>The vacuum pump, RV series</strong>, is an oil pump with a two-stage high-efficiency system, providing stability, high flow, ultra-high vacuum (minimum 1.5mtorr), and low noise during operation. It also has various features, such as a three-stage vacuum selection and an overheat protection device, and can be used for strong acid organic solvents.</li>
</ol>
</div>Usage and Reservation Rules<div class="ed_model01 clearfix">
<ol>
<li class="ed_txt">Download the operation manual to watch. <a href="/userfiles/ordch/files/20231018132747639.pdf" rel="noreferrer noopener" target="_blank" title="(click to download)(pdf)(Open New Windows)">(click to download)</a></li>
<li class="ed_txt">Contact the center's technical staff to learn the precautions and to be qualified to make reservations.</li>
<li class="ed_txt">You must make online reservations through the "<a href="https://ircbooking.nycu.edu.tw/IRC/irc_booking/index_en.aspx" rel="noreferrer noopener" target="_blank" title="Instrument Reservation System(Open New Windows)">Instrument Reservation System</a>" beofre use.</li>
<li class="ed_txt"><strong>If you want to use other solvents than water and alcohol, you must check with the center staff whether you can use that before operating the instrument.<br />
(Please provide the SDS chemical safety data sheet, also known as "safety data sheet.")</strong></li>
<li class="ed_txt">To maintain the cleanliness and function of the machine, please empty the condensate tank solvent after use for the benefit other users.</li>
<li class="ed_txt">The simple operation manual is placed on site, please read it carefully before use.</li>
</ol>
</div>References<div class="ed_model17 clearfix ed_list">
<ul>
<li class="d-flex"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;">Easy Operation Manual</span> </span>
<ul class="d-flex">
<li><a href="/userfiles/ordch/files/20231018132929896.pdf" rel="noreferrer noopener" target="_blank" title="Easy Operation Manual(Open New Windows)"><img alt="Easy Operation Manual" src="/userfiles/file/pdf.png" /> </a></li>
</ul>
</li>
</ul>
</div>Related Links<div class="ed_model01 clearfix">
<div class="ed_txt"><a href="https://www.thermofisher.com/tw/zt/home.html" rel="noreferrer noopener" target="_blank" title="ThermoFisher(Open New Windows)"><img alt="ThermoFisher" src="/userfiles/ordch/images/20231018102723132.png" /></a></div>
</div>https://www.nycu.edu.tw/ord/en/app/machine/image?module=machine&detailNo=1167349780943212544&init=YSpeedVachttps://ord.edu.tw/ord/en/app/artwebsite/view?module=artwebsite&id=2023&serno=da69be39-dc4e-48d6-9168-7c96b2dbdb6eInstrument List of YM Campushttps://www.nycu.edu.tw/ord/en/app/machine/list?module=machine&id=2060Concentrator and Dryer