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Image System

exchangealt 中文
  • Update Date：2025-04-11
  • Units：Instrumentation Resource Center
[YangMing] Luminescence/Fluorescence Image System - GE ImageQuant
Image Capture and Analysis System AI600, AI680, ImageQuant 800
  • Luminescence/Fluorescence Image System
  • Brand Model
    • GE Amersham Imager-600/680: machine K, L, and M
    • GE ImageQuant 800: machine N
  • Instrument Location: Yang-Ming Campus
    • Room 467, 4F. Biomedical Engineering Building (machine M*)
    • Room 710, Biomedical Building (machine N)
    • Room B02, B1F. Shou-ren Building (machine K*), (machine L*)
      * The machine has a fluorescent function.
  • Term of use: Reservation required
    • Free of charge for on-campus use; please contact the center staff for off-campus use.
  • Reservation >>> Instrument Reservation System
  • Contact center staff：
Amersham Imager 680 luminescence, UV light, fluorescence image capture system is an upgraded version of ImageQuant LAS-4000 series, suitable for imaging detection and analysis of protein and DNA samples of gels and Western Blot transfer membranes.
  • Detection range includes Chemiluminescence, Fluorescence (UV, red, green, blue), and white light imaging.
  • The hardware has white light, UV light, and fluorescence. Using image capture from different light sources, samples can be analyzed to determine the protein or nucleic acid concentration under the following situations.
Model, light source and Epi-RGB emission filter information
Model Light source Emission Filter
(for Epi-RGB)
AI600
AI680		 White Epi/Trans-illumination Light: 470-635 nm
UV Trans-illumination Light: 312 nm
Blue Epi Light: 460 nm
Green Epi Light: 520 nm
Red Epi Light: 630 nm		 —
—
Cy2: 525 BP20
Cy3: 605 BP40
Cy5: 705 BP40
ImageQuant 800 Luminescence source only —
Imaging methods, sample types, and suitable trays and accessories
Imaging methods Sample Type Suitable Tray and Accessories
Chemiluminescence (cold light) Membrane Black Tray or Black Tray + White Insert
Colorimetric (visible light - upper side illumination)
Epi- illumination		 Membrane, gel Black Tray + White Insert or White Trans Tray
Colorimetric (visible light - transmitted illumination from the bottom)
Trans- illumination		 Gel White Trans Tray + Diffuser Board
Fluorescence (fluorescence -upper side illumination)
Epi-RGB		 Membrane, gel Black Tray
Fluorescence (ultraviolet light-transmitted illumination from the bottom) UV Gel UV Trans Tray (transparent)
(L machine only, reservation time is limited to office hours from 08:00-17:00)

The functions of IQTL software can be used for analysis:
  1. 1D electrophoresis gel quantification
  2. Western blot quantification
  3. Colony Count
  4. Array analysis (MicroArray)
※This machine must be booked via the Instrument Reservation System before Use.
Each person can make a maximum of 1 reservation, with a limit of 0.5 or 1 hour per reservation. Please make changes as soon as possible since multiple users use this device. Failure to make a reservation or a reservation under a different name will be penalized per the regulations.

Turning on the instrument
  1. The first user daily should press the On/Off button in front fo the device to start the instrument.
  2. After self-diagnosis, the main screen will be displayed. Press Continue to enter the operation interface.
  3. The instrument has an automatic sleep mechanism. If you are not the first user daily and the instrument is in the sleep mode, press Resume at the lower right corner of the instrument's main screen.
  4. Please wait for the CCD cooling to be completed and for the status to from orange to green (as shown below) before you start to capture images.
    Power-on instructions orange lightPower-on instructions green light
    When the orange light in the lower left corner turns to green, you can use it.
Instrument use 
  1. Please use the matching tray and accessories correctly according to the sample type. (For samples other than WB membrane, please contact us)
  2. When using, do not throw the Sample to avoid contamination of the surrounding environment.
  3. If you find that the previous user has caused environmental contamination, please make sure to record it in the log book to avoid any misunderstanding before use.
  4. After use, clean and wipe the front and back of the board and the tray.
  5. At the end of the operation, if you are not the last user of the day, you do not need to turn off the power of the host computer, the instrument has an automatic sleep mechanism.
  6. It is strictly prohibited to use gloves; EtBr samples are also prohibited.
Instrument shutdown
The last user of the day (after 5pm) should press the On/Off button (in front of the device) to turn off the instrument after use.
  • GE AI600 Simple Operation Manual (Chinese version)
  • GE AI600 User Manual (English version)
  • GE AI800 User Manual (English version)
Instrument operation video
"Operation Introduction" Play 20:28

Analysis software
  1. ImageQuant TL
  2. Multi Gauge 18:36
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Instrument List of YM Campus Image System
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