Spectrophotometer
中文- Update Date：2025-01-15
- Units：Instrumentation Resource Center
[YangMing] NanoDrop Spectrophotometer: ND-1000
- Brand/model: NanoDrop ND-1000
- Instrument Location: Yang-Ming Campus
- Room 465, 4F. Biomedical Engineering Building (B machine)
- Room 701, 7F. Biomedical Building (A machine), (E machine)
- Room 212, 2F. Department of Nursing (F machine)
- Room 615, 6F. Library, Information & Research Building (C machine)
- Room B02, B1 Floor, Shou-ren Building (D machine)
- Term of Use: Available anytime
- Free of charge for NYCU users. Off-campus users, please contact the center before use.
- Only nucleic acid samples, no proteins!
- Contact center staff：
- Extension：62382
- Email：ycirc@nycu.edu.tw
The ND-1000 spectrophotometer is NanoDrop's first product. It uses the surface tension characteristics of liquids and only requires a small amount of sample to measure. A fixed optical diameter (1mm and 0.2mm) is pulled out in the detection stage (pedestal) through the contact of the upper and lower arms to detect the absorption value, which achieves the advantages of rapid, micro-volume, and high-concentration measurements and is free of cuvettes, capillary tubes, and other consumables. This design is patented and popular worldwide, allowing scientists to conduct research more efficiently. (Web link).
- The ND-1000 uses a high-energy xenon flash lamp, which can provide complete spectrum detection of 220-750nm. There is no need to warm it up, and it can be used immediately after turning it on use. Equipped with a high-sensitivity CCD array detector, the absorbance detection value can be as high as 75Abs, i.e., dsDNA concentration of 2~3700ng/ul. Mainly, purified nucleic acid can be detected directly without dilution.
- The homogeneity of the samples to be tested is the highest requirement of ND-1000. Generally, samples should be homogenized by a vortex mixer or pipetting several times before detection. To avoid genomic DNA samples breaking due to the above action, you can heat the sample at 55ﾟC for about one minute to homogenize before detection.
- Note: Only nucleic acid samples can be used! No protein samples!
- Different detection modes:
- Nucleic Acid – Absorption spectrum, 230nm, 260nm, 280nm absorption values (converted to 10mm aperture absorption values), 260/280 ratio, 260/230 ratio, and nucleic acid concentration.
- UV-Vis – Absorption values and spectra for all wavelengths between 190 and 850nm (presented as 1mm path length absorptions).
| Sample Type | Minimum concentration | Maximum concentration | Reproducibility (more than five repetitions) |
|---|---|---|---|
| Nucleic acid | 2 ng/μl | 3700 ng/μl (dsDNA) 3000 ng/μl (RNA) 2400 ng/μl (ssDNA) |
|
| Pure BSA | 0.10 mg/ml | 100 mg/ml |
|
Example: dsDNA Measurement
- Click Nucleic Acid on the main screen, follow the pop-up dialog boxes of the software (confirm that the table is clean, and put 1ul of secondary water on the pedestal, put down the upper arm, and press OK) to complete the connection of computer and instrument.
- Prepare the solvent of the DNA sample (be sure to confirm that the DNA is dissolved in secondary water, TE buffer, or which set of kit's elution buffer), and take out 1.5~2 ul on the pedestal, put down the upper arm and then press Blank.
- Select Sample Type DNA-50 in the upper right corner, enter the sample name in the Sample ID position, mix the sample, take out 1.5~2 ul and place it on the pedestal, put down the upper arm, and press Measure. "Download Demonstration Video"
- Result:
The DNA concentration is calculated as 65.003 x 50 = 3250.1.
- The 260/280 value between 1.8 and 2.0 usually means relatively pure DNA (the value will vary depending on the DNA composition).
- If the 260/230 value is higher than 260/280 and is between 1.8 and 2.2, it usually means fewer residual pollutants are in the purification process.
- If you want to view the absorption values of other wavelengths between 220 and 340nm, you can use the mouse to drag the straight line at 230nm or enter other values on the right λ
Result Report: All samples detected can be obtained by clicking Show Report. This report can be saved as a ndj file and opened using Excel. For results from different days, you can select “Data Viewer à Import Samples” and select the results you want to put in the same report to generate a new report. "Download Demonstration Video"
- This machine is available for use at any time.
- After sample detection, please use the Kimwipe to wipe the pedestal and clean RO water to check the background value. The background value must be within ±0.04. Otherwise, it must be cleaned again and re-read until it is within the range to be considered as the end of the cleaning. Write down the ratio of A260 and A280 in the registration book.
Notes
- Only nucleic acid samples can be used! No protein samples!
- Use lens wiping paper (such as Kimwipe) to wipe the pedestal immediately after detection. First, take one piece to absorb the liquid on the upper and lower countertops, then fold the surface of the laboratory wipe that has absorbed the sample to the inside, fold it four times, and wipe the countertop multiple times in one direction (DNA wipe 5 times). " Download demo video》
- One drop of the same fluid can only be used once for detection. If you want to repeat measurements of the same sample, please wipe off the previous drop and replace it with a new one.
- Basically, 1~2ul of the nucleic acid sample can be measured. The volume requirement may vary according to the characteristics of each liquid volume. In principle, it should not be more than 2ul. Please use the 2ul pipette to avoid insufficient volume, which may lead to the failure of the complete formation of the liquid column.
- Whenever the software pops up an error message, please read it carefully and follow the instructions to troubleshoot. The most common situation is that the liquid column must be formed correctly during detection. Observe with your naked eyes to check whether the liquid column is connected to the pedestals or if bubbles are in the sample. Pull up the upper arm, wipe off the drop, and repeat the detection, increasing the sample volume to 2ul if necessary.
- The correct liquid column should be:
- Do not use samples containing Hydrofluoric Acid (HF); other non-corrosive liquids can be used.
- The black wire above the instrument is an optical fiber; please do not pull it during use.
- Ensure the table and the instrument are kept clean at all times.
- Calibrate the instrument regularly.
- For additional FAQs, please refer to the ND-1000 Support.
- ND-1000 User Manual
- Instructional video "How to operate ND-1000"
![[ND-1000 User Manual]](/userfiles/file/pdf.png)
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