Concentrator and Dryer
中文- Update Date：2025-01-15
- Units：Instrumentation Resource Center
[YM Campus] Vacuum Concentrator System: SpeedVac
- Vacuum Concentrator System
- Brand/Model: Thermo SPD121P SpeedVac* Kits
- Instrument Location: Yang-Ming Campus
- Room 438-1, Biomedical Engineering Building
- Term of Use:
- Contact the center's technical staff to explain the dos and don'ts to be qualified to make reservations.
- Free of charge for NYCU users. Off-campus users, please contact the center before using it.
- Reservation >>> Instrument Reservation System
- Contact center staff：
- Extension：62382
- Email：ycirc@nycu.edu.tw
The Principle of Concentrating centrifuge
Concentration centrifugation is a process that uses heat energy to evaporate the liquid solvent and concentrate precious samples. When the solvent evaporates, the remaining crystals are the substances originally dissolved in the solvent, such as purified DNA, RNA, or proteins.
To evaporate solvents with high boiling points, low pressure can be used to reduce the boiling point. For example, water has a boiling point of 100°C at atmospheric pressure (1.0 bar), but at low pressure (0.48 bar), the boiling point drops to about 80°C. When using high-efficiency vacuum pumps to reach extremely low pressure (<<< 0.001 bar), water will evaporate quickly at room temperature.
The high vacuum environment not only improves efficiency but also eliminates oxygen and other free radicals in the air, preventing protein oxidation or denaturation during the concentration process. To prevent sample loss due to sudden boiling under high vacuum conditions, centrifugal force can effectively push the sample to the bottom of the sample bottle. Additionally, a good rotor design can maintain the maximum surface area of the liquid by tilting it at a 45° angle with the centrifugal axis.
When the solvent contains corrosive or harmful substances, a condensation system is required between the sample volatilization tank and the vacuum pump. This system helps to collect the condensed volatilized gas at a low temperature, reducing health and safety risks for users and prolonging the life of the vacuum pump.
Features:
The concentrator centrifuge includes the host (SPD121P), condensate tank, and vacuum pump.
Concentration centrifugation is a process that uses heat energy to evaporate the liquid solvent and concentrate precious samples. When the solvent evaporates, the remaining crystals are the substances originally dissolved in the solvent, such as purified DNA, RNA, or proteins.
To evaporate solvents with high boiling points, low pressure can be used to reduce the boiling point. For example, water has a boiling point of 100°C at atmospheric pressure (1.0 bar), but at low pressure (0.48 bar), the boiling point drops to about 80°C. When using high-efficiency vacuum pumps to reach extremely low pressure (<<< 0.001 bar), water will evaporate quickly at room temperature.
The high vacuum environment not only improves efficiency but also eliminates oxygen and other free radicals in the air, preventing protein oxidation or denaturation during the concentration process. To prevent sample loss due to sudden boiling under high vacuum conditions, centrifugal force can effectively push the sample to the bottom of the sample bottle. Additionally, a good rotor design can maintain the maximum surface area of the liquid by tilting it at a 45° angle with the centrifugal axis.
When the solvent contains corrosive or harmful substances, a condensation system is required between the sample volatilization tank and the vacuum pump. This system helps to collect the condensed volatilized gas at a low temperature, reducing health and safety risks for users and prolonging the life of the vacuum pump.
Features:
The concentrator centrifuge includes the host (SPD121P), condensate tank, and vacuum pump.
- The SPD series host is designed to concentrate biological and non-biological samples. It features TEFLON industrial-grade anti-corrosion coatings and precision rotors for different sample containers.
- The condensate tank, RVT series, provides a low-temperature waste liquid recycle function, ensuring that the vacuum pump is not corroded by organic substances. It uses Germany's Danfoss compressor, has a temperature as low as -50°C, and uses an environmentally friendly refrigerant.
- The vacuum pump, RV series, is an oil pump with a two-stage high-efficiency system, providing stability, high flow, ultra-high vacuum (minimum 1.5mtorr), and low noise during operation. It also has various features, such as a three-stage vacuum selection and an overheat protection device, and can be used for strong acid organic solvents.
- Download the operation manual to watch. (click to download)
- Contact the center's technical staff to learn the precautions and to be qualified to make reservations.
- You must make online reservations through the "Instrument Reservation System" beofre use.
- If you want to use other solvents than water and alcohol, you must check with the center staff whether you can use that before operating the instrument.
(Please provide the SDS chemical safety data sheet, also known as "safety data sheet.") - To maintain the cleanliness and function of the machine, please empty the condensate tank solvent after use for the benefit other users.
- The simple operation manual is placed on site, please read it carefully before use.
- Easy Operation Manual
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