Link to Content Area

NYCU Office of Research and Development

::: SITEMAP HOME REGULATIONS FORMS NYCU 中文
  • font-small
  • font-medium
  • font-large
Search
open menu

Analyzers

exchangealt 中文
  • Update Date：2025-01-15
  • Units：Instrumentation Resource Center
[YangMing] Seahorse XFe24 Extracellular Flux Analyzer
Seahorse Bioenergy Metabolism Analyzer
  • Seahorse XFe24 Extracellular Flux Analyzer
  • Instrument Location: Yang-Ming Campus
    • Room 710, Biomedical Building 
  • Term of Use: Prior reservation required
    • Charging:  follow the charging standards below
  • Booking>>> Instrument Reservation System
  • Contact center staff：
Seahorse XFe24 Extracellular Flux Analyzer can instantly detect the rate of oxygen consumption and acid production during cell metabolism.

The unique patented design of this device can continuously detect changes in cell energy without touching or harming cells by sensing the concentration of oxygen and hydrogen ions in the surrounding environment. It is the only device in the world that can simultaneously detect cellular aerobic and anaerobic respiration, covering the instrumentation of overall energy metabolism. The device is stable and intuitive, making it accessible for studying energetics.

This device has several applications:
  1. Translational Medical Research - The device can analyze human samples such as WBC, platelet, fibroblast, and muscle biopsy to evaluate the relationship between various diseases and energy, establishing a database of the correlation between human energy and diseases. Researchers have published preliminary research results on AIDS, neurodegenerative diseases, depression, diabetes, and glutaric acidemia and have even published them in international journals. It requires a small amount of specimen and can analyze multiple samples simultaneously, making it a breakthrough tool that connects basic research and clinical medicine.
  2. Diabetes, obesity, and various metabolic syndromes - The device can help researchers understand the use of different nutrients by organisms at the basic research level, thereby analyzing the pathogenesis of various metabolic syndromes or quickly screening drugs with therapeutic potential. At the clinical level, assessing the characteristics of patients' own metabolic systems will contribute to developing disease diagnosis and treatment strategies, establishing a clinical metabolism database, and improving the diversity of related examination services.
  3. Aging and degenerative neurological diseases - The device can help researchers understand the operation of mitochondria and the intracellular metabolic system, which is closely related to many neurodegenerative diseases such as Parkinson's disease, Huntington's disease, Alzheimer's disease, and physical degeneration caused by aging. Defects in mitochondria or related pathways can destroy the balance of energy supply within cells, causing cells to lose function or die, ultimately leading to various aging and disease.
  4. Oxidative stress, ischemia- reperfusion-induced cellular damage - These factors mainly come from free radicals (reactive oxygen species, ROS) produced by mitochondria. This type of molecule can destroy the structure of various biomolecules in cells and cause them to lose their functions, making it the primary source of intracellular stress. Understanding the function of mitochondria helps researchers explain ROS's source and operating mechanism.
  5. Cancer Research - The device can help study the relationship and importance of metabolic systems and cancer and accelerate the development of cancer treatment strategies. The Warburg effect points out that the metabolic system of cancer cells prefers to use glycolysis to produce lactic acid as the primary energy compared to normal cells. This metabolic characteristic is closely related to the aggressiveness, proliferation, metastasis speed, growth ability, and cancerization process of cancer cells. The lactic acid produced is essential for cancer cells to affect surrounding normal cells.
  6. Toxicity research and metabolism-modulating drug development - The device can assist researchers in drug development by detecting mitochondrial/metabolic system toxicity early in drug screening to avoid investing R&D costs in researching toxic and unsuitable drugs. It can also help to screen compounds with specific metabolic regulation abilities for subsequent development of metabolism-related therapeutic drugs.
  7. Cell quality monitoring - The device can be used to observe the metabolic characteristics of the cell line itself as a "biological fingerprint." By observing the sensitive metabolic system, researchers can understand the differentiation or growth changes of cells over time, which can affect the results of experiments. This system offers clear judgment criteria and sensitivity, unlike traditional methods that rely on observation of cell life and appearance.
Features:
  1. Our technology can detect mitochondrial aerobic metabolism and glycolysis anaerobic metabolism pathways simultaneously.
  2. It can analyze various samples, including adherent cells, suspended cells, organelles, and tissue samples.
  3. The microwell plate design requires only a small number of cells, speeding up the research process.
  4. The highly sensitive optical detection system does not require any reagents.
  5. The entire process is non-contact and non-destructive, allowing for sample recycling and providing flexibility.
First-time users, please note
  • If you have no experience using it, please contact the center first to arrange teaching by the original product specialist to ensure the experiment goes smoothly.
  • If you have experience using it, you can visit the Instrument Reservation System to make reservations.
Usage and Reservation Rules
  1. Visit the Instrument Center's website to reserve a time slot up to 2 weeks in advance. Each individual can book up to 2 slots, each a maximum of 4 hours. Reservations are available from 00:00 to 23:30.
  2. Users are responsible for purchasing their consumables.
  3. All necessary information must be recorded in the registration book before using the machine. Please ensure you get your samples quickly after use to avoid any damage, for which you will be held responsible.
  4. Bring your own flash drive for data backup. Due to limited space on the computer hard drive, the administrator may periodically delete data to ensure proper operation of the instrument. Users must manage their own data storage, as the center does not assume responsibility for data storage.
  5. If you encounter instrument malfunction before use, please inform the center staff and document it in the usage registration book to determine responsibility accurately.
  6. Only individuals with a reservation may use the instruments; no queue jumping is allowed. The person who made the reservation must be the actual user.
  7. Do not delay the following user's appointment. A reservation is considered abandoned if it is over an hour late, and after three such instances, instrument use will be revoked.
  8. Cancellations must be made at least 24 hours in advance if the reservation is unused.
  • Experimental preparation process
  • Software operation instructions–Chinese
  • XFe software User Guide–Eng
  • Experimental Operation Manual
  • XF software analysis software (fill in the basic information to download)
Charging standards
Instrument Unit NYCU faculty and students Cooperative educational units Other units
Seahorse XFe24 Every 4 hours
(less than 4 hours will be counted as 4 hours)		 NT$250 NT$375 NT$500
  • Starting from February 1, 2011, fees will be charged according to the standard.
  1. If you have the following qualifications, you will be charged the same fee as NYCU faculty and students:
    • Branch school
    • Joint-Appointed faculty 
    • Affiliated hospitals 
    • Projects are done in cooperation with and under the control of the University.
  2. If you have the following qualifications, the fee will be the same as that of a cooperative educational unit:
    • Adjunct faculty
    • Teaching hospitals (Veterans General Hospital, Taipei City Hospital, Far Eastern Memorial Hospital, Cheng Hsin General Hospital, etc.)
    • University System of Taiwan (UST)
    • Companies participate in the academia-industry cooperation center of NYCU
Instrument List of YM Campus Other Analyzers
Related Image(s)：
Back
gotop