Microtomes
中文- Update Date：2025-01-15
- Units：Instrumentation Resource Center
[YangMing] Cryostat Microtome - epredia (Thermo)
- Cryostat Microtome
- Brand: epredia (Thermo)
- Model: CryoStar NX50, NX70
- Instrument Location: Yang-Ming Campus
- Room 465, Biomedical Engineering Building (I Machine-NX70)
- Room 710, Biomedical Building (J Machine-NX70)
- Room 615, Library, Information & Research Building (H Machine-NX70)
- Room B02, Shou-Ren Building (F Machine-NX50) (K Machine-NX70)
- Term of use: Reservation required
- Free of charge for NYCU students and faculties. For off-campus users, please contact the center staff before use.
- Reservation >>> Instrument Reservation System
- Contact center staff：
- Extension：62382
- Email：ycirc@nycu.edu.tw
- The scope of application of this machine is as follows:
- For fresh tissue sections.
- Slicing range 0.5-500 um
- Specimen head temperature set to -45°C
Before use:
- Please register your and your instructor's names in the registration book. Ensure the machine is in good condition and the cryochamber is clean.
- Quick freezing of the specimen:
- Activate the “Cryobar” button on the control panel.
- Use a freezing compound (such as OCT) to fix the specimen onto the Object Disc (specimen holder)
- Place the Object Disc (specimen holder) on the Cryobar to freeze the specimen.
- Clean the used Object Disc (specimen holder) and return it to its original location if you borrow it from the center.
- Place back the protective cover of the Blade Holder.
- Clean the remaining residual glue and specimen inside the cryochamber.
- Tighten the handle clamps.
- Close the window of cryochamber.
- Press Stand-by on the control panel.
- Reservations must be made online using the Instrument Reservation System before use.
- Reservations can be made as early as two weeks in advance. Each user can book two sessions in 2 weeks, with a maximum of five hours for each session.
- For general operation training, please contact the center's staff for demonstration.
- Please use the original labeled Object Disc (specimen chuck), and do not take the borrowed Object Disc and related items away from the Center.
- Do not turn off the main switch of the machine.
- Please keep it clean and functional for all students and faculty.
- Please read the user's manual before use.
- Please do not leave your belongings inside the cryochamber after use, and take away the used blades and waste.
- Please bring your own anti-roll plate!
- NX50 Manual (English version)
- NX70 Manual (English version)
- Operation Instruction Video (editing)
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